Now showing items 11-20 of 2338
Cloning and characterization of a DNA polymerase β gene from Trypanosoma cruzi
A gene coding for a DNA polymerase β from the Trypanosoma cruzi Miranda clone, belonging to the TcI lineage, was cloned (Miranda Tcpolβ), using the information from eight peptides of the T. cruzi β-like DNA polymerase ...
Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form
Chagas disease, caused by the protozoan Trypanosoma cruzi, is a major parasitic disease that affects millions of people in America. However, despite the high impact of this disease on human health, no effective and ...
Deletion of DNA sequences of using a polymerase chain reaction based approach
(Pontificia Universidad Católica de Valparaíso, 2006)
Endogenous overexpression of an active phosphorylated form of DNA polymerase β under oxidative stress in Trypanosoma cruzi
(Public Library of Science, 2018)
© 2018 Rojas et al. Trypanosoma cruzi is exposed during its life to exogenous and endogenous oxidative stress, leading to damage of several macromolecules such as DNA. There are many DNA repair pathways in the nucleus and ...
Colorimetric detection of DNA polymerase activity after sodium dodecyl sulfate polyacrylamide gel electrophoresis
A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- ...
Both XPA and DNA polymerase eta are necessary for the repair of doxorubicin-induced DNA lesions
(ELSEVIER IRELAND LTDCLARE, 2012)
Doxorubicin (DOX) is an important tumor chemotherapeutic agent, acting mainly by genotoxic action. This work focus on cell processes that help cell survival, after DOX-induced DNA damage. In fact, cells deficient for XPA ...
Inhibition of Taq DNA Polymerase and Human Topoisomerase I by Resveratrol Derivatives
(Bentham Science Publishers, 2017-05)
Background: DNA polymerases and topoisomerases are proteins that play a vital part in DNA metabolism such as replication, transcription, recombination, and chromosome segregation during mitosis. For this reason, these ...