Now showing items 1-10 of 2338
Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase
(Associação Brasileira de Divulgação Científica, 1998-10-01)
For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) ...
Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors
DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase ...
Purification and characterization of a β-like DNA polymerase from Trypanosoma cruzi
A DNA polymerase was purified to near homogeneity from Trypanosoma cruzi epimastigotes. This preparation had a major polypeptide of 50 kDa and a minor band of 45 kDa. SDS-PAGE studies and a novel colorimetric activity gel ...
INHIBITION OF DNA-POLYMERASE ALPHA AND REVERSE-TRANSCRIPTASE ACTIVITIES BY HPA-23
(Inst Tecnologia ParanaCuritiba-paranaBrasil, 1991)
Inhibition of Taq DNA polymerase by catalpol.
(C M B Association, 2004-09)
DNA polymerases have recently emerged as important cellular targets for chemical intervention in the development of anti-cancer agents. This report describes a PCR assay as a method to investigate the action mechanism of ...
DNA from oral bacteria by sodium hydroxide-paper method suitable for polymerase chain reaction
In the oral cavity, we can find a complex mixture of microorganisms, commensals, and pathogens. The studies of normal oral microbiota, as well as the studies of much oral pathology (e.g., caries, periodontitis), involve ...
DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression
(National Academy Of Sciences, 2016-07)
DNA damage tolerance facilitates the progression of replication forks that have encountered obstacles on the template strands. It involves either translesion DNA synthesis initiated by proliferating cell nuclear antigen ...
INHIBITION OF THE WHEAT-GERM DNA POLYMERASE-A ACTIVITY BY THE ANTIVIRAL DRUG HPA-23
(Kluwer Academic PublDordrechtHolanda, 1993)
Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR.
The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus ...