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Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
(IOP Publishing Ltd, 2017-09)
In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and ...
Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
(Elsevier, 2022-02)
Hypothesis: Polarity in polyelectrolyte multilayers (PEMs) may vary from the inner to the top layers of the film as the charge compensation of the layers is more effective inside the PEMs than in outer layers. Doxorubicin ...
β-sheet to α-helix conversion and thermal stability of β-Galactosidase encapsulated in a nanoporous silica gel
(Academic Press Inc Elsevier Science, 2019-01-01)
The effect on protein conformation and thermal stability was studied for β-Galactosidase (β-Gal) encapsulated in the nanopores of a silicate matrix (Eβ-Gal). Circular dichroism spectra showed that, compared with the enzyme ...
Fluorescence of dicyanovinyl julolidine in a room-temperature ionic liquid
(Elsevier Science, 2006-08)
Steady-state and time-resolved emission of 9-dicyanovinyl julolidine (DCVJ) in 1-butyl 3-methyl imidazolium tetrafluoroborate ([bmim][BF4]) were studied. The emission spectrum of DCVJ, obtained after properly subtracting ...
Canopy active fluorescence spectrum tracks ANPP changes upon irrigation treatments in soybean crop
(Elsevier Science Inc., 2021-09)
Accurate estimation of aerial net primary production (ANPP) using remotely acquired data is one of the main challenges in both environmental monitoring and precision agriculture. Reflectance-based techniques have been ...
Eosin y triplet state as a probe of spatial heterogeneity in microcrystalline cellulose
(Wiley Blackwell Publishing, Inc, 2012-07)
The photophysical behavior of eosin Y adsorbed onto microcrystalline cellulose was evaluated by reflectance spectroscopy, steady-state fluorescence spectroscopy and laser induced time-resolved luminescence. On increasing ...
FAD binding properties of a cytosolic version of Escherichia coli NADH dehydrogenase-2
(Elsevier Science, 2014-03)
Respiratory NADH dehydrogenase-2 (NDH-2) of Escherichia coli is a peripheral membrane-bound flavoprotein. By eliminating its C-terminal region, a water soluble truncated version was obtained in our laboratory. Overall ...
Photophysics of Xanthene Dyes at High Concentrations in Solid Environments: Charge Transfer Assisted Triplet Formation
(Wiley Blackwell Publishing, Inc, 2018-09)
The photophysical behavior of two xanthene dyes, Eosin Y and Phloxine B, included in microcrystalline cellulose particles is studied in a wide concentration range, with emphasis on the effect of dye concentration on ...
Optical studies of single-tryptophan B. licheniformis β-lactamase variants
(Elsevier Science, 2010-10)
β-lactamases (penicillinases) are important complicating factors in bacterial infections and excellent theoretical and experimental models in protein structure, dynamics and evolution. Bacillus licheniformis exo-small ...
Fluorescence of 4-aminophthalimide in supercritical CO2–ethanol mixtures
(Elsevier Science, 2004-10)
Steady-state and time-resolved fluorescence studies of 4-aminophthalimide (AP) in neat supercritical CO2 and supercritical CO2–ethanol mixtures at 35 and 45 °C are presented. In neat CO2, the emission maximum of AP shifts ...