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Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A
(Univ Federal Santa MariaSanta MariaBrasil, 2009)
Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR
(SPRINGER FRANCE, 2008)
For obtaining accurate and reliable gene expression results it is essential that quantitative real-time RT-PCR (qRT-PCR) data are normalized with appropriate reference genes. The current exponential increase in postgenomic ...
A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
(2016-01-01)
Groundnut ringspot virus (GRSV) is a tospovirus species transmitted in a circulative propagative manner by several thrips species. Traditionally, GRSV is detected by enzyme-linked immunosorbent assay (ELISA) and reverse ...
Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
(Sociedade Brasileira de Genética, 2007-01-01)
T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, ...
Piezoelectric biosensors for real-time monitoring of hybridization and detection of hepatitis C virus
(Elsevier B.V., 2004-05-01)
The piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus (HCV) in serum. The gold electrodes on either rough or smooth surface crystals were modified ...
Detecção do vírus da cinomose canina por diferentes métodos de one-step RT-qPCR
(2016-09-01)
Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and ...
References Genes For Qrt-pcr In Guarana (paullina Cupana Var. Sorbilis)
(SOC BOTANICA SAO PAULOSAO PAULO, 2015)
Canine visceral leishmaniasis and Rhipicephalus sanguineus: evaluation and comparison of classical techniques
(2022-02-01)
The aim of this study was evaluating the association and correlation between the diagnostics tests used for Leishmania spp. detection in dogs and ticks. We evaluated 99 dogs and 990 Rhipicephalus sanguineus. In dogs, we ...