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Real time PCR. Application in dengue studies.
(2011-11-09)
PCR (polymerase chain reaction) is a routinely used tool in every diagnostic and research laboratory. This technique has
been used in detection of mutations and pathogens, forensic investigation, and even is the base tool ...
Real-time PCR strategy for the identification of Trypanosoma cruzi discrete typing units directly in chronically infected human blood
(Elsevier B.V., 2017)
© 2017 Elsevier B.V. The protozoan Trypanosoma cruzi is the causative agent of Chagas disease, a major public health problem in Latin America. This parasite has a complex population structure comprised by six or seven major ...
A Real-time PCR assay for detection of low pneumocystis jirovecii levels
(Frontiers Media, 2022)
Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii. To do so, two primer sets were designed, targeting the family ...
Detection of Helicobacter pylori by Real-Time PCR for 16s rRNA in Stools of NonInfected Healthy Children, Using ELISA Antigen Stool Test as the Gold Standard
(Wiley-Blackwell, 2016)
BackgroundWe previously detected Helicobacter pylori infection by stool antigen ELISA assay in 33-41% of asymptomatic Chilean children between 2-3 years of age, of which 11-20% had a transient infection and 21-22% a ...
Simultaneous detection of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus using real-time PCR and high resolution melting analysis
(Elsevier, 2014)
In this study, the real-time PCR assays were combined with high resolution melting (HRM) analysis for
the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle
virus (CGRMV) ...
Discrete typing units of Trypanosoma cruzi detected by real-time PCR in Chilean patients with chronic Chagas cardiomyopathy
(Elsevier B.V., 2018)
© 2018 Elsevier B.V. Chagas disease is a major public health problem in Latin America and has spread to other countries due to immigration of infected persons. 10–30% of patients with chronic Chagas disease will develop ...
Patterns of Piscirickettsia salmonis load in susceptible and resistant families of Salmo salar
(Elsevier, 2015)
The pathogen Piscirickettsia salmonis produces a systemic aggressive infection that involves several organs and tissues in salmonids. In spite of the great economic losses caused by this pathogen in the Atlantic salmon ...
Detection of Dengue Virus in Department of Bolivar, Colombia, 2012- 2013, Real-Time PCR.
(Rajasansi, India: Journal Of Advances In Biotechnology, 2014., 2019-11-12)
To assess the presence of RNA from dengue virus by Real Time Polymerase Chain Reaction with Reverse Transcriptase (qRT-PCR), in anti-dengue IgM seronegative serum samples obtained from symptomatic patients with less than ...
Circulation of influenza in backyard productive systems in central Chile and evidence of spillover from wild birds
(Elsevier, 2018-05)
Backyard productive systems (BPS) are recognized as the most common form of animal production in the world. However, BPS frequently exhibit inherent biosecurity deficiencies, and could play a major role in the epidemiology ...
Evaluation of Nifurtimox Treatment of Chronic Chagas Disease by Means of Several Parasitological Methods
(American Society for Microbiology, 2013-09)
Currently, evaluation of drug efficacy for Chagas disease remains a controversial issue with no consensus. In this work, we evaluated
the parasitological efficacy of Nifurtimox treatment in 21 women with chronic Chagas ...