Now showing items 1-10 of 1348
Incidência de vírus em videiras determinada por meio da técnica de RT-PCR em tempo real.
(In: MOSTRA DE ENSINO, EXTENSÃO E PESQUISA, 17., 2015. Lajeado. Anais...Lajeado: Ed. da Univates, 2015. p. 386., 2015)
Detecção do vírus da cinomose canina por diferentes métodos de one-step RT-qPCR
Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and ...
Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I
(Japan Soc Vet Sci, 2018-04-01)
A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. ...
Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR
(SPRINGER FRANCE, 2008)
For obtaining accurate and reliable gene expression results it is essential that quantitative real-time RT-PCR (qRT-PCR) data are normalized with appropriate reference genes. The current exponential increase in postgenomic ...
Evaluation of Quantitative RT-PCR Using Nonamplified and Amplified RNA
(LIPPINCOTT WILLIAMS & WILKINS, 2010)
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is a standard assay in molecular medicine for gene expression analysis. Samples from incisional/needle biopsies, laser-microdissected tumor cells and ...
Improved Detection of Citrus psorosis virus and Coat Protein-Derived Transgenes in Citrus Plants: Comparison Between RT-qPCR and TAS-ELISA
(Wiley Blackwell Publishing, Inc, 2015-12)
Citrus is one of the most economically important fruit crops in the world. Citrus psorosis is a serious disease affecting mainly oranges and mandarins in Argentina and Uruguay. The causal agent is Citrus psorosis virus ...
Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma
(Biomed Central Ltd., 2009-03-23)
Background: HER-2 gene testing has become an integral part of breast cancer patient diagnosis. The most commonly used assay in the clinical setting for evaluating HER-2 status is immunohistochemistry (IHC) and fluorescence ...
Canine Distemper Virus Detection By Different Methods Of One-step Rt-qpcrDetecção Do Vírus Da Cinomose Canina Por Diferentes Métodos De One-step Rt-qpcr
(Universidade Federal de Santa Maria, 2016)
Defining suitable reference genes for RT-qPCR analysis on human sertoli cells after 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure
Quantitative real-time RT-PCR (qPCR) has proven to be a valuable molecular technique to quantify gene expression. There are few studies in the literature that describe suitable reference genes to normalize gene expression ...
A new set of reference genes for RT-qPCR assays in the yeast Dekkera bruxellensis
(Canadian Science Publishing, Nrc Research PressOttawaCanadá, 2012)