Now showing items 1-10 of 5714
Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase
(Associação Brasileira de Divulgação Científica, 1998-10-01)
For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) ...
Purification and characterization of a β-like DNA polymerase from Trypanosoma cruzi
A DNA polymerase was purified to near homogeneity from Trypanosoma cruzi epimastigotes. This preparation had a major polypeptide of 50 kDa and a minor band of 45 kDa. SDS-PAGE studies and a novel colorimetric activity gel ...
RRN7 PROTEIN, AN RNA POLYMERASE I TRANSCRIPTION FACTOR, IS REQUIRED FOR RNA POLYMERASE II-DEPENDENT TRANSCRIPTION DIRECTED BY CORE PROMOTERS WITH A HOMOLD BOX SEQUENCE
(AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2011)
Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
(Sociedad de Biología de Chile, 2006)
INHIBITION OF DNA-POLYMERASE ALPHA AND REVERSE-TRANSCRIPTASE ACTIVITIES BY HPA-23
(Inst Tecnologia ParanaCuritiba-paranaBrasil, 1991)
A bumpy road for RNA polymerase II
(Nature Publishing Group, 2015-05)
The identification of a second regulatory checkpoint controlling RNA polymerase II elongation near the poly(A) site of protein-coding genes reveals an additional level of complexity in the modulation of eukaryotic ...
Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors
DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase ...
Inhibition of Taq DNA polymerase by catalpol.
(C M B Association, 2004-09)
DNA polymerases have recently emerged as important cellular targets for chemical intervention in the development of anti-cancer agents. This report describes a PCR assay as a method to investigate the action mechanism of ...
Deletion of DNA sequences of using a polymerase chain reaction based approach
(Pontificia Universidad Católica de Valparaíso, 2006)
The Polymerase Chain Reaction