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Variably improved microbial source tracking with digital droplet PCR
(Pergamon-Elsevier Science Ltd, 2019-08-01)
This study addressed whether digital droplet PCR (ddPCR) could improve sensitivity and specificity of human-associated Bacteroidales genetic markers, BacHum and B. theta, and their quantification in environmental and fecal ...
Utilização de marcadores de rDNA-PCR e tDNA-PCR para tipagem de isolados clínicos de Pseudomonas aeruginosa
(Universidade Federal de Pernambuco, 2014)
Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
(2011)
Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. ...
Evaluation of the analytical and diagnostic performance of a digital droplet polymerase chain reaction (ddPCR) assay to detect Trypanosoma cruzi DNA in blood samples
Background: The recent development of novel Polymerase Chain Reaction (PCR) technologies that confer theoretical advantages over quantitative PCR has considerable potential in the diagnosis of low load infections, such as ...
Characterization of human intestinal bifidobacteria using competitive PCR and PCR-TTGE
(BLACKWELL, 2006-01)
In this study, a competitive PCR was developed to estimate the quantity of bifidobacteria in human faecal samples using two 16S rRNA gene Bifidobacterium genus-specific primers, Bif164f and Bif662r. A PCR-temporal temperature ...