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Cryopreservation at-80A degrees C of Agaricus blazei on rice grains
(Springer, 2011-12-01)
The preservation of Agaricus blazei is generally done by mycelial subculturing, but this technique may cause genetic degenerations. Despite this, there is not an efficient protocol established to preserve this fungus and ...
Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
(Pontificia Universidad Católica de Valparaíso<BR>Facultad de Recursos Naturales<BR>Escuela de Ciencias del Mar, 2011)
Effects of the cryopreservation process on dog sperm integrity
(Brazilian Coll Animal Reproduction, 2020-01-01)
Sperm cryopreservation has become an indispensable tool in reproductive biology. However, frozen/thawed semen has a short lifespan due to loss of sperm cell integrity. To better understand which sperm cell structures are ...
Cytosine methylation of sperm DNA in horse semen after cryopreservation
(2016-09-15)
Semen processing may contribute to epigenetic changes in spermatozoa. We have therefore addressed changes in sperm DNA cytosine methylation induced by cryopreservation of stallion semen. The relative amount of 5-methylcytosine ...
Conditioned medium: a new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells
(Wiley-Blackwell, 2017-03-01)
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples, and implementation of new in vitro studies. This work parts from the ...
Different extenders in the cryopreservation of bovine epididymal spermatozoa
(Elsevier B. V., 2015)
The objective of this study was to evaluate the effects of two different egg yolk extenders incubated with or without Sperm Talp on the motility and plasma membrane integrity of cryopreserved bovine epididymal spermatozoa ...
PARADOXICAL EFFECT OF QUERCETIN ANTIOXIDANT ON GOAT SPERM PARAMETERS AFTER CRYOPRESERVATION
(Cryo Letters, 2020-05-01)
BACKGROUND: Some antioxidants have been used in semen extenders to reduce adverse effects caused by excessive reactive oxygen species (ROS) production. The study was carried out to assess the effect of quercetin (QC) ...
Specific protocols of controlled ovarian stimulation for oocyte cryopreservation in breast cancer patients
(2018-12-01)
Background Fertility preservation is an important concern in breast cancer patients. In the present investigation, we set out to create a specific protocol of controlled ovarian stimulation (cos) for oocyte cryopreservation ...