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Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
(Bio-protocol, 2019-08)
Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses ...
Stem CellsCélulas-tronco
(HCPA/FAMED/UFRGS, 2008)
Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
(Springer, 2014-06)
The dynamics of in situ 2D HeLa cell quasi-linear and quasi-radial colony fronts in a standard culture medium is investigated. For quasi-radial colonies, as the cell population increased, a kinetic transition from an ...
Insights into Stem Cell Factor chemotactic guidance of neural crest cells revealed by a real-time directionality-based assay
(Elsevier Gmbh, 2012-05)
The extracellular environment through which neural crest cells (NCCs) translocate and differentiate plays a crucial role in the determination of cell migration and homing. In the trunk, NCC-derived melanocyte precursor ...
Cell-to-cell transmission is the main mechanism supporting bovine viral diarrhea virus spread in cell culture
(American Society for Microbiology, 2019-01)
After initiation of an infective cycle, spread of virus infection can occur fundamentally in two different ways: (i) viral particles can be released into the external environment and diffuse through the extracellular space ...
Magnetic-Activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
(Humana Press, 2018)
This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, ...
Cell-based interventions to halt autoimmunity in type 1 diabetes mellitus
(Wiley, 2013-01)
Type 1 diabetes mellitus (T1DM) results from death of insulin-secreting b cells mediated by self-immune cells, and the consequent inability of the body to maintain insulin levels for appropriate glucose homeostasis. ...
Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
(Nature Publishing Group, 2019-05)
Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early ...
Modeling active cell movement with the Potts model
(Frontiers Research Foundation, 2018-06-20)
In the last decade, the cellular Potts model has been extensively used to model interacting cell systems at the tissue-level. However, in early applications of this model, cell movement was taken as a consequence of membrane ...
Pulsing Dendritic Cells with Whole Tumor Cell Lysates
(Springer, 2014-03)
One of the strategies employed in immunotherapy for cancer is to use of ex vivo-generated dendritic cells (DC) pulsed with tumor antigens. Several approaches have been used to obtain and load tumor antigens in DC. One such ...