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Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
(2009-04-01)
Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A ...
Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation
(1999-02-01)
Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrp1p is one of several ...
Probing the binding of the coumarin drugs using peptide fragments of DNA gyrase B protein
(2005-05-01)
Bacterial DNA gyrase, has been identified as the target of several antibacterial agents, including the coumarin drugs. The coumarins inhibit the gyrase action by competitive binding to the ATP-binding site of DNA gyrase B ...
CHARACTERISTICS OF ZINC-BINDING TO HUMAN RED-BLOOD-CELL MEMBRANES
(Wiley-Blackwell, 1994-01-01)
The objective of the present study was to standardize the analysis of zinc binding on human red blood cell (RBC) membranes in 20 normal adults. The displacement studies revealed that at the maximal stable zinc concentration ...
BINDING of SEX-HORMONE-BINDING GLOBULIN (SHBG) TO TESTICULAR MEMBRANES and SOLUBILIZED RECEPTORS
(Elsevier B.V., 1992-11-01)
Sex-hormone-binding globulin (SHBG) binds to a specific protein on the surface of prostate, epididymis, and a human breast cancer cell line (MCF-7), and is internalized by these cells. the present study demonstrated specific ...
Probing the Specificity of Binding to the Major Nuclear Localization Sequence-binding Site of Importin-alpha Using Oriented Peptide Library Screening
(Amer Soc Biochemistry Molecular Biology Inc, 2010-06-25)
Importin-alpha is the nuclear import receptor that recognizes the classic monopartite and bipartite nuclear localization sequences (cNLSs), which contain one or two clusters of basic amino acids, respectively. Different ...
A new biophysics approach using photoacoustic spectroscopy to study the DNA-ethidium bromide interaction
(Springer, 2002-06-01)
We have examined the binding processes of ethidium bromide interacting with calf thymus DNA using photoacoustic spectroscopy. These binding processes are generally investigated by a combination of absorption or fluorescence ...
The Influence of Solutes on the Enthalpy/Entropy Change of the Actinomycin D Binding to DNA: Hydration, Energy Compensation and Long-Range Deformation on DNA
(Amer Chemical Soc, 2011-07-21)
The effects of the changes in the temperature and in the water chemical potential on the energetic of the actinomycin D (ACTD) interaction with natural DNA are studied. At reduced water chemical potential, induced by the ...
Structure of thrombin complexed with selective non-electrophilic inhibitors having cyclohexyl moieties at P1
(2000-03-25)
The crystal structures of five new non-electrophilic β-strand-templated thrombin active-site inhibitors have been determined bound to the enzyme. Four co-crystallize with hirugen and inhibitor isomorphously to produce ...