Now showing items 1-10 of 369
Adsorption of antiphospholipid antibodies on affinity magnetoliposomes
(Elsevier Science BvAmsterdamHolanda, 2008)
Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to ...
Evaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of Toxoplasma gondii and Neospora caninum in maned wolves (Chrysocyon brachyurus)
Use of serological tests in the diagnosis of infectious diseases in wild animals has several limitations, primarily the difficulty of obtaining species-specific reagents. Wild canids, such as maned wolves (Chrysocyon ...
Affinity Maturation Increases the Stability and Plasticity of the Fv Domain of Anti-protein Antibodies
The somatic mutations accumulated in variable and framework regions of antibodies produce structural changes that increase the affinity towards the antigen. This implies conformational and non covalent bonding changes at ...
Preparation of N2, N2,7-trimethylguanosine affinity columns
2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. ...
Generation of sequence-specific, high affinity anti-DNA antibodies.
(American Society for Biochemistry and Molecular Biology, 2001-12)
By taking advantage of the extreme stability of a protein-DNA complex, we have obtained two highly specific monoclonal antibodies against a predetermined palindromic DNA sequence corresponding to the binding site of the ...