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Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum
(Nature America Inc, 2001-10-01)
An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. These ...
Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum
(Nature America Inc, 2001-10-01)
An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. These ...
Purification and characterization of an iron-activated alkaline phosphatase produced by rhizopus microsporus var. Microsporus under submerged fermentation using rye flour
(2020-07-01)
Current researches have been carried out to find microorganisms that can produce enzymes for different biotechnological purposes. Among the enzymes, the microbial phosphatases, responsible for hydrolyzing phosphoric acid ...
Alkaline phosphatases: In silico study on the catalytic effect of conserved active site residues using human placental alkaline phosphatase (plap) as a model protein
(American Chemical Society, 2020-12)
The metalloenzymes from the alkaline phosphatase (AP) superfamily catalyze the hydrolysis and transphosphorylation of phosphate monoesters. The role of several amino acids highly conserved in the active site of this family ...
Construction of an alkaline phosphatase-liposome system: a tool for biomineralization study
(Elsevier B.V., 2002-09-01)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper. we standardize a method for ...