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A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
(Blackwell Publishing, 2004-01-01)
Aims: Detection of Xylella fastidiosa in citrus plants and insect vectors.Methods and Results: Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp ...
Nicotiana tabacum as an experimental host for the study of plant-Xylella fastidiosa interactions
(2000-08-01)
Xylella fastidiosa causes citrus variegated chlorosis (CVC). Information generated from the X. fastidiosa genome project is being used to study the underlying mechanisms responsible for pathogenicity. However, the lack of ...
A putative twin-arginine translocation system in the phytopathogenic bacterium Xylella fastidiosa
(Canadian Science Publishing, Nrc Research Press, 2011-02-01)
The twin-arginine translocation (Tat) pathway of the xylem-limited phytopathogenic bacterium Xylella fastidiosa strain 9a5c, responsible for citrus variegated chlorosis, was explored. The presence of tatA, tatB, and tatC ...
Recombinant expression and characterization of a Xylella fastidiosa cysteine protease differentially expressed in a nonpathogenic strain
(Blackwell Publishing, 2006-08-01)
Xylella fastidiosa is a xylem-limited, Gram-negative bacterium responsible for citrus variegated chlorosis (CVC) in sweet oranges. In the present study, we present the recombinant expression, purification and characterization ...
Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene
(Sociedade Brasileira de Genética, 2003-01-01)
The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase ...
alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization
(Academic Press Ltd Elsevier B.V. Ltd, 2009-09-01)
Xylella fastidiosa is a xylem-restricted plant pathogen that causes a range of diseases in several and important crops. Through comparative genomic sequence analysis many genes were identified and, among them, several ...
Molecular models for shikimate pathway enzymes of Xylella fastidiosa
(Elsevier B.V., 2004-07-30)
The Xylella fastidiosa is a bacterium that is the cause of citrus variegated chlorosis (CVC). The shikimate pathway is of pivotal importance for production of a plethora of aromatic compounds in plants, bacteria, and fungi. ...
Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
(Sociedade Brasileira de Fruticultura, 2008-03-01)
A primeira bactéria fitopatogênica a ter seu genoma totalmente seqüenciado foi detectada e isolada em diferentes hospedeiros em diferentes regiões geográficas. Embora seja causadora de doenças em culturas economicamente ...
The Complex Biogeography of the Plant Pathogen Xylella fastidiosa: Genetic Evidence of Introductions and Subspecific Introgression in Central America
(2014-11)
The bacterium Xylella fastidiosa is a plant pathogen with a history of economically damaging introductions of subspecies to regions where its other subspecies are native. Genetic evidence is presented demonstrating the ...
Genomics-based design of defined growth media for the plant pathogen Xylella fastidiosa
(Elsevier B.V., 2003-02-14)
Based on the genetic analysis of the phytopathogen Xylella fastidiosa genome, five media with defined composition were developed and the growth abilities of this fastidious prokaryote were evaluated in liquid media and on ...