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In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence
(FUNPEC-EDITORA, 2010)
The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence ...
Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer
(Cambridge University Press, 2005-11-01)
As an important step in the nuclear transfer (NT) procedure, we evaluated the effect of three different treatments for oocyte activation on the in vitro and in vivo developmental capacity of bovine reconstructed embryos: ...
Somatic cell nuclear transfer : history, future and potential application in the bubaline species
(Universidad Nacional del Nordeste, 2011)
Introduction: In 1914, Hans Spemann demonstrated that salamanders had pluripotent nuclei up to the 16-cell stage (Spemann, 1938). In this classic study, the author utilized a hair from his son to constrict a newly fertilized ...
Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
(Pontificia Universidad Católica de Valparaíso, 2011)
A journey through horse cloning
(Csiro Publishing, 2017-12)
Interest in equine somatic cell nuclear transfer technology has increased significantly since the first equid clones were produced in 2003. This is demonstrated by the multiple commercial equine cloning companies having ...
6-Dimethylaminopurine and cyclohexamide are mutagenic and alter reproductive performance and intrauterine development in vivo
(Funpec-editora, 2015-01-01)
The compounds 6-dimethylaminopurine (6-DMAP) and cyclohexamide (CHX) are currently used to stimulate the development of embryos produced by nuclear transfer in the production of cloned mammals with a great deal success. ...
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer
(2020-08-01)
The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. ...
Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer
(Funpec-editora, 2015-01-01)
Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the ...
Aberrant expression of E-cadherin and beta-catenin proteins in placenta of bovine embryos derived from somatic cell nuclear transfer
(CSIRO Publishing, 2012-01-01)
Abnormal placental development is common in the bovine somatic cell nuclear transfer (SCNT)-derived fetus. In the present study, we characterised the expression of E-cadherin and beta-catenin, structural proteins of adherens ...
Somatic cell nuclear transfer is associated with altered expression of angiogenic factor systems in bovine placentomes at term
(Funpec-editora, 2010-01-01)
Low efficiency of somatic cell cloning by nuclear transfer has been associated with alterations of placental vascular architecture. Placental growth and function depend on the growth of blood vessels; VEGF-A and bFGF are ...