Now showing items 1-10 of 382
Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
Serine proteases have wide application in leather, food, meat and soap powder industries, among others. There are a lot of methodologies to obtain them in large quantities but most of them are expensive or contaminating. ...
Characterization, subsite mapping and N-terminal sequence of miliin, a serine-protease isolated from the latex of Euphorbia milii
(Elsevier B.V., 2013-04-01)
Miliin is a serine protease purified from the latex of Euphorbia milii. This work reports the effect of pH and temperature on the catalytic activity of miliin, using fluorescence resonance energy transfer (FRET) substrates. ...
Antimicrobial activity of the venom of the Peruvian snake Bothrops oligolepis: involvement of a metalloprotease, a type-C lectin and a serine-protease.
(In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Resumos. Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Resumo 1437-1., 2015)
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii
(Bentham Science Publ Ltd, 2008-07-01)
Miliin, a new thiol-dependent serine protease purified from the latex of Euphorbia milii possesses a molecular weight of 79 kDa, an isoelectric point of 4.3 and is optimally active at 60 degrees C in the pH range of and ...
Edema induced by a crotalus durissus terrificus venom serine protease (Cdtsp 2) involves the PAR pathway and PKC and PLC activation
Snake venom serine proteases (SVSPs) represent an essential group of enzymatic toxins involved in several pathophysiological effects on blood homeostasis. Some findings suggest the involvement of this class of enzymatic ...
Immobilized analogues of sunflower trypsin inhibitor-1 constitute a versatile group of affinity sorbents for selective isolation of serine proteases
(ELSEVIER SCIENCE BV, 2009)
Sunflower trypsin inhibitor-1 (SFI-1), a natural 14-residue cyclic peptide, and some of its synthetic acyclic variants are potent protease inhibitors displaying peculiar inhibitory profiles. Here we describe the synthesis ...
BjSP, a novel serine protease from Bothrops jararaca snake venom that degrades fibrinogen without forming fibrin clots
Snake venom serine proteases (SVSPs) are commonly described as capable of affecting hemostasis by interacting with several coagulation system components. In this study, we describe the isolation and characterization of ...
Purification and characterization of a new alkaline serine protease from the thermophilic fungus Myceliophthora sp.
(Elsevier B.V., 2011-11-01)
This work reports the purification of a novel alkaline protease enzyme from a putative new thermophilic fungus Myceliophthora sp. The molecular weight of the enzyme was determined as 28.2 kDa by using MALDI-TOF MS and it ...