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Hla-mapper: An application to optimize the mapping of HLA sequences produced by massively parallel sequencing procedures
(2018-09-01)
A challenging task when more than one HLA gene is evaluated together by second-generation sequencing is to achieve a reliable read mapping. The polymorphic and repetitive nature of HLA genes might bias the read mapping ...
Nonoverlapping Clone Pooling for High-Throughput Sequencing
(Ieee Computer Soc, 2013-09-01)
Simultaneously sequencing multiple clones using second-generation sequencers can speed up many essential clone-based sequencing methods. However, in applications such as fosmid clone sequencing and full-length cDNA sequencing, ...
Mitogenome assembly from genomic multiplex libraries: comparison of strategies and novel mitogenomes for five species of frogs
(Wiley-Blackwell, 2016-05-01)
Next-generation sequencing continues to revolutionize biodiversity studies by generating unprecedented amounts of DNA sequence data for comparative genomic analysis. However, these data are produced as millions or billions ...
Draft Genome Sequence of Geobacillus sp. Isolate T6, a Thermophilic Bacterium Collected from a Thermal Spring in Argentina
(American Society for Microbiology, 2015-07)
Geobacillus sp. isolate T6 was collected from a thermal spring in Salta, Argentina. The draft genome sequence (3,767,773 bp) of this isolate is represented by one major scaffold of 3,46 Mbp, a second one of 207 Kbp and ...
KIR2DL4 genetic diversity in a Brazilian population sample: implications for transcription regulation and protein diversity in samples with different ancestry backgrounds
(2021-06-01)
KIR2DL4 is an important immune modulator expressed in natural killer cells; HLA-G is its main ligand. We have characterized the KIR2DL4 genetic diversity by considering the promoter, all exons, and all introns in a highly ...
The Streptomyces leeuwenhoekii genome: de novo sequencing and assembly in single contigs of the chromosome, circular plasmid pSLE1 and linear plasmid pSLE2
(BioMed Central, 2015)
Background: Next Generation DNA Sequencing (NGS) and genome mining of actinomycetes and other microorganisms is currently one of the most promising strategies for the discovery of novel bioactive natural products, potentially ...
Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
(Brazilian Soc Plant Breeding, 2021-09)
A prerequisite for commercial release of a transgenic crop variety inArgentina and other countries in the world is the identification and sequenceanalysis of the transgene(s) insertion site in the plant genome. Southern ...
HLA-A promoter, coding, and 3′UTR sequences in a Brazilian cohort, and their evolutionary aspects
(2019-02-01)
HLA-A is the second most polymorphic locus of the human leucocyte antigen (HLA) complex encoding a key molecule for antigen presentation and NK cell modulation. Many studies have evaluated HLA-A variability in worldwide ...
Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)
(Sociedade Brasileira de Genética, 2006-01-01)
The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence ...