Now showing items 1-10 of 2449
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
Human interferon B1 ser17: Coding DNA synthesis, expression, purification and characterization of bioactive recombinant protein
A protocol to produce large amounts of bioactive homogeneous human interferon β1 expressed in Escherichia coli was developed. Human interferon β1 ser17 gene was constructed, cloned and subcloned, and the recombinant protein ...
Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein
(BioMed Central, 2010-10)
Background: Multiple HIV-1 intersubtype recombinants have been identified in human populations. Previous studies from our lab group have shown that the epidemic in Argentina is characterized by the high prevalence of a ...
Aqueous extraction of maize endosperm: Insights for recombinant protein hosts based on downstream processing
(Elsevier Sci LtdOxfordInglaterra, 2005)
Transcription factor engineering in CHO cells for recombinant protein production
(Taylor and Francis Ltd, 2019)
The continuous increase of approved biopharmaceutical products drives the development of more efficient recombinant protein expression systems. Chinese hamster ovary (CHO) cells are the mainstay for this purpose but have ...
Production of recombinant enzymes of wide use for research
(Pontificia Universidad Católica de Valparaíso, 2006)
Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
(Oxford University Press, 2014-12)
The mismatch repair (MMR) system maintains genome integrity by correcting replication associated errors and inhibiting recombination between divergent DNA sequences. The basic features of the pathway have been highly ...