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Variably improved microbial source tracking with digital droplet PCR
(Pergamon-Elsevier Science Ltd, 2019-08-01)
This study addressed whether digital droplet PCR (ddPCR) could improve sensitivity and specificity of human-associated Bacteroidales genetic markers, BacHum and B. theta, and their quantification in environmental and fecal ...
A quantitative TaqMan PCR assay for the detection of Mycoplasma suis
(WILEY-BLACKWELL, 2011)
Aim: To develop a TaqMan probe-based, highly sensitive and specific quantitative PCR (qPCR) assay for the detection and quantification of Mycoplasma suis in the blood of pigs. Methods and Results: Primers and probes specific ...
The performance of four molecular methods for the laboratory diagnosis of congenital toxoplasmosis in amniotic fluid samples
(Belo Horizonte, 2013)
Introduction
Toxoplasmosis may be life-threatening in fetuses and in immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence of IgM and IgG anti-Toxoplasma gondii antibodies; ...
Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR
(SPRINGER FRANCE, 2008)
For obtaining accurate and reliable gene expression results it is essential that quantitative real-time RT-PCR (qRT-PCR) data are normalized with appropriate reference genes. The current exponential increase in postgenomic ...
Optimization of Methods for Detecting Hepatitis A Virus in Food
(Springer Verlag, 2017)
Development of a New Genus-Specific Quantitative Real-Time PCR Assay for the Diagnosis of Scrub Typhus in South America
(2022)
Scrub typhus is a potentially severe rickettsiosis, caused by Orientia tsutsugamushi in the Asia-Pacific region. Recently, however, two distinct pathogens, “Candidatus Orientia chuto” and “Candidatus Orientia chiloensis”, ...