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Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins
(Elsevier, 2003-03)
The variable genes of monoclonal antibody (mAb) B10, specific for the C-terminal region of the eukaryotic ribosomal P protein, have been cloned as a single-chain Fv fragment (scFv) and expressed in Escherichia coli. The ...
Solvents to fragments to drugs: MD applications in drug design
(Molecular Diversity Preservation International, 2018-12)
Simulations of molecular dynamics (MD) are playing an increasingly important role in structure-based drug discovery (SBDD). Here we review the use of MD for proteins in aqueous solvation, organic/aqueous mixed solvents ...
Cultivation of Pichia pastoris carrying the scFv anti LDL (−) antibody fragment. Effect of preculture carbon source
(Sociedade Brasileira de Microbiologia, 2017-07)
Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions ...
The promoter of the sunflower HD-Zip protein gene Hahb4 directs tissue-specific expression and is inducible by water stress, high salt concentrations and ABA
(Elsevier Ireland, 2005-08)
In the present work, we have analysed the promoter region of the sunflower nuclear gene Hahb4, encoding an homeodomain-leucine zipper protein involved in water stress responses. This region is represented in two different ...
Spectroscopic and Theoretical Study of CuI Binding to His111 in the Human Prion Protein Fragment 106-115
(American Chemical Society, 2016-03)
The ability of the cellular prion protein (PrPC) to bind copper in vivo points to a physiological role for PrPC in copper transport. Six copper binding sites have been identified in the nonstructured N-terminal region of ...
A region of the SARS-CoV-2 spike protein functionally interacts with the human α7 nicotinic receptor
(Sociedad Argentina de Biofísica, 2021)
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The binding of the viral spike protein (S) to angiotensin converting enzyme 2 in host cells is crucial for ...
Solvent mimicry with methylene carbene to probe protein topography
(American Chemical Society, 2015-09)
The solvent accessible surface area (SASA) of the polypeptide chain plays a key role in protein folding, conformational change, and interaction. This fundamental biophysical parameter is elusive in experimental measurement. ...
Atomically-detailed folding simulation of the B domain of staphylococcal protein A from random structures
(National Academy of Sciences, 2003-09)
The conformational space of the 10–55 fragment of the B-domain of staphylococcal protein A has been investigated by using the electrostatically driven Monte Carlo (EDMC) method. The ECEPP/3 (empirical conformational energy ...
The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae
(Portland Press, 2012-09)
PDK1 (phosphoinositide-dependent protein kinase 1) phosphorylates and activates PKA (cAMP-dependent protein kinase) in vitro. Docking of the HM (hydrophobic motif) in the C-terminal tail of the PKA catalytic subunits on ...
Expression profile and distribution of Annexin A1, A2 and A5 in human semen
(Wiley Blackwell Publishing, Inc, 2019-01)
Annexins (ANXAs) have been identified in different seminal components mainly by proteomic methods. The presence and distribution of Annexin A1, A2 and A5 (ANXA1, ANXA2, ANXA5) in human semen was analysed and the corresponding ...