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Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis
(Reproductive Healthcare Ltd, 2022)
Research question: Does resveratrol exert a potent inhibitory effect on the development of endometriosis by interfering with some pivotal processes?Design: In-vitro cultures of primary endometriotic stromal cells, immortalized ...
Estradiol stimulates cell proliferation via classic estrogen receptor-alpha and G protein-coupled estrogen receptor-1 in human renal tubular epithelial cell primary cultures
(Academic Press Inc Elsevier Science, 2019-04)
This work was aimed to determine the effect of 17β-estradiol (17βE) on cell proliferation in human renal tubular epithelial cells (HRTEC) isolated from kidneys from pediatric subjects, as well as the role of estrogen ...
Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
(Bio-protocol, 2019-08)
Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses ...
GABAA receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity
(Pergamon-Elsevier Science Ltd, 2010-02)
Toxicity risk assessment for chemical-induced human health hazards relies mainly on data obtained from animal experimentation, human studies and epidemiology. In vitro testing for acute toxicity based on cytotoxicity assays ...
A simple strategy for culturing morphologically-conserved rat hypothalamic tanycytes
(Springer, 2017-08)
Hypothalamic tanycytes are specialized bipolar ependymal cells that line the floor of the third ventricle. Given their strategic location, tanycytes are believed to play several key functions including being a selective ...
A long-term in vitro culture of bovine epithelial cells on collagen rafts
(Wiley Blackwell Publishing, Inc, 2021-02)
In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free-floating type I collagen hydrogels (rafts) at an air-liquid interface (ALI). ...
Imaging amyloid precursor protein in vivo: An axonal transport assay
(Humana Press, 2012-08)
Fusion of fluorescent probes to axonally transported proteins represents an established approach that enables live imaging of axonal transport. In this approach, in vivo examination of fluorescent particle dynamics provides ...
Deciphering the role of miR-71 in echinococcus multilocularis early development in vitro
(Public Library of Science, 2019-12)
Echinococcosis represents a major public health problem worldwide and is considered a neglected disease by the World Health Organization. The etiological agents are Echinococcus tapeworms, which display elaborate developmental ...
Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection
(Elsevier Science, 2021-05)
Bovine viral diarrhea virus (BVDV) and bovine gammaherpesvirus 4 (BoHV-4) infect the uterus of cattle, being responsible for huge economic losses. Most of the pathogenesis of BoHV-4 in the bovine reproductive tract has ...
Involvement of GSK3/β-catenin in the action of extracellular ATP on differentiation of primary cultures from rat calvaria into osteoblasts
(Wiley-liss, div John Wiley & Sons Inc., 2018-06-22)
Modulation of purinergic receptors play an important role in the regulation of osteoblasts differentiation and bone formation. In this study, we investigated the involvement of the GSK3/βcatenin signaling in the action of ...