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Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes
(SPRINGER, 2009)
The possibility of obtaining transplantable oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal failures. As reconstructive material this in vitro epithelia would be ...
Human breast tumor slices as an alternative approach to cell lines to individualize research for each patient
(Lippincott Williams & Wilkins, 2012-07-01)
There are several breast cancer experimental models including cell lines, which are commonly used due to ease of handling and storage. However, the continued propagation of cell lines and distribution among laboratories ...
Effects of Escherichia Coli subtilase cytotoxin and shiga toxin 2 on primary cultures of human renal tubular epithelial cells
(Public Library of Science, 2014-01)
Shiga toxin (Stx)-producing Escherichia coli (STEC) cause post-diarrhea Hemolytic Uremic Syndrome (HUS), which is the most common cause of acute renal failure in children in many parts of the world. Several non-O157 STEC ...
Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor
(2016-01-01)
with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell ...
Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors
(2013-04-01)
Breast cancer is the most common type of cancer among women worldwide. Research using breast cancer cell lines derived from primary tumors may provide valuable additional knowledge regarding this type of cancer. Therefore, ...
Comparative analysis of salmon cell lines and zebrafish primary cell cultures infection with the fish pathogen piscirickettsia salmonis
(MDPI, 2021)
Piscirickettsia salmonis is the etiologic agent of piscirickettsiosis, a disease that causes
significant losses in the salmon farming industry. In order to unveil the pathogenic mechanisms of
P. salmonis, appropriate ...
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
(Spandidos Publ Ltd, 2017-03-01)
The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation ...
A comprehensive characterization of cell cultures and xenografts derived from a human verrucous penile carcinoma
(2016-08-01)
This study aimed to establish and characterize primary cell cultures and xenografts derived from penile carcinoma (PeCa) in order to provide experimental models for cellular processes and efficacy of new treatments. A ...
Culture of osteogenic cells from human alveolar bone: A useful source of alkaline phosphatase
(Elsevier B.V., 2007-11-01)
The aim of this study was to obtain membrane-bound alkaline phosphatase from osteoblastic-like cells of human alveolar bone. Cells were obtained by enzymatic digestion and maintained in primary culture in osteogenic medium ...
Diagnosis and treatment of hep-2 cells contaminated with mycoplasmaDiagnóstico e tratamento de células hep-2 contaminadas por micoplasma
(2021-02-01)
Contamination of primary and cell cultures by mycoplasmas is one of the main economic and biological pitfalls in basic research, diagnosis and manufacture of biotechnological products. It is a common issue which may be ...