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Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
(Bio-Protocol LLC, 2017-12)
We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously ...
Polyhydroxybutyrate-based nanocomposites with cellulose nanocrystals and bacterial cellulose
(Springer, 2016-09-24)
Polyhydroxybutyrate (PHB) films nanoreinforced with hydrolyzed cellulose nanocrystals (CNC) and bacterial cellulose (BC) were prepared by solvent casting. The influence of different cellulose nanoparticles content (2, 4 ...
Biodegradable double-layer films based on biological resources: Polyhydroxybutyrate and cellulose
(John Wiley & Sons Inc, 2007-10)
A novel biocomposites double‐layer films using polyhydroxybutyrate (PHB) and cellulose paper were produced. The biocomposites were prepared by the solvent‐casting method (with chloroform). Films of the blends were characterized ...
Visualising the concept of metabolic regulation in bacteria: a simple laboratory experiment on polyhydroxybutyrate storage
(Taylor & Francis, 2020-05)
Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how ...
Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
(Oxford University Press, 2006-11)
Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase ...
Plant growth-promoting bacterium Pseudomonas fluorescens FR1 secrets a novel type of extracellular polyhydroxybutyrate polymerase involved in abiotic stress response in plants
(Springer, 2018-10)
Objectives: Identification of novel microbial factors contributing to plant protection against abiotic stress. Results: The genome of plant growth-promoting bacterium Pseudomonas fluorescens FR1 contains a short mobile ...
Understanding the intracellular-to-extracellular localization switch of polyhydroxybutyrate polymerase in pseudomonas backgrounds as a microevolutionary process
(Academic Press Ltd - Elsevier Science Ltd, 2018-11)
After gene duplication, paralogous genes evolve independently, and consequently, the new proteins encoded by these duplicated genes are exposed to changes in their subcellular location. Although there are increasing evidence ...
Pseudomonas extremaustralis sp. nov. a polyhydroxybutyrate-producer isolated from Antarctic environments
(Springer, 2009-12)
A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the ...
Poly(3-hydroxybutyrate) influences biofilm formation and motility in the novel Antarctic species Pseudomonas extremaustralis under cold conditions
(Springer Tokyo, 2011-09)
Polyhydroxyalkanoates (PHAs) are highly reduced bacterial storage compounds that increase fitness in changing environments. It has previously shown that polyhydroxybutyrate (PHB) accumulation is essential during the growth ...
Phylogenetic distribution and evolutionary pattern of an alfa-proteobacterial small RNA gene that controls polyhydroxybutyrate accumulation in Sinorhizobium meliloti
(Academic Press Inc Elsevier Science, 2016-03)
It has become clear that sRNAs play relevant regulatory functions in bacteria. However, a comprehensive understanding of their biological roles considering evolutionary aspects has not been achieved for most of them. Thus, ...