Buscar
Mostrando ítems 1-7 de 7
Photo-activatable surfaces for cell migration assays
(Wiley Vch Verlag, 2013-12)
A simple material platform for advanced cell migration studies is presented. It relies on surfaces modified with photo-activatable adhesion peptides and two light exposure steps to independently trigger attachment and ...
Quantifying transcription factor-DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy
(Nature Publishing Group, 2017-07)
Probing transcription factor (TF)-DNA interactions remains challenging in complex in vivo systems such as mammalian embryos, especially when TF copy numbers and fluorescence background are high. To address this difficulty, ...
A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging
(American Chemical Society, 2016-06)
A coumarin fluorophore and an oxazine photochrome can be integrated within the same molecular skeleton and connected covalently to a secondary antibody. Illumination of the antibody-dyad conjugate at an appropriate activation ...
Photoactivatable Fluorophores for Super-Resolution Imaging Based on Oxazine Auxochromes
(American Chemical Society, 2012-03)
We synthesized five fluorophore-photochrome dyads designed to switch reversibly between nonfluorescent and fluorescent isomers under optical control. These compounds pair an oxazine photochrome to a biphenyl, fluorene, ...
Xantenos y porfirinas, moléculas fotoactivables empleadas en el control de insectos plagaXantenes and porphyrins, photoactivatable molecules used to control insect pests
(Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2011-12)
Se analizaron dos sensibilizadores fotodinámicos, la Floxina B y la Hematoporfirina IX, como fotoinsecticidas de moscas plagas. Ambas sustancias adicionadas al alimento de las larvas presentaron alta toxicidad. La ...
Photoswitchable fluorescent diheteroarylethenes: substituent effects on photochromic and solvatochromic properties
(Royal Society of Chemistry, 2014-01)
Photoswitchable fluorescent diheteroarylethenes are promising candidates for applications in super-resolution molecular localization fluorescence microscopy thanks to their high quantum yields and fatigue-resistant ...
Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction
(American Society for Biochemistry and Molecular Biology, 2013-08)
As recently described by our group, plasma membrane calcium ATPase (PMCA) activity can be regulated by the actin cytoskeleton. In this study, we characterize the interaction of purified G-actin with isolated PMCA and examine ...