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A novel PCR-RFLP assay for the detection of the single nucleotide polymorphism at position+1440 in the human CXCR2 gene
(Springer, 2007-10-01)
We designed a novel PCR-RFLP assay to genotype for the CXCR2 +1440 (G/A) single nucleotide polymorphism, which provides a simple, low-cost, practical, and reproducible method. Allele frequencies in healthy Brazilian ...
Detecção do polimorfismo de base única (rs2227307) no gene interleucina 8 humano usando PCR-RFLP
(2009-11-01)
Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms (SNPs) in the human IL8 gene have been investigated in many disease association studies. We have ...
Allelic Variant Analysis of the DRB3 Gene by PCR-RFLP in Mediterranean Buffaloes Breed
(Int Buffalo Information Ctr, 2013-01-01)
A cultivation-independent PCR-RFLP assay targeting oprF gene for detection and identification of Pseudomonas spp. in samples from fibrocystic paediatric patients
(Elsevier Science, 2015-05)
Species-specific genetic markers are crucial to develop faithful and sensitive molecular methods for the detection and identification of Pseudomonas aeruginosa (Pa). We have previously set up a PCR-RFLP protocol targeting ...
Typing class I HLA-A gene using a nested PCR-RFLP procedure
(Associação Brasileira de Divulgação Científica (ABRADIC), 2005-06-01)
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by ...
Use of PCR-RFLP (Polymerase Chain Reaction - Restricted Fragment Length Polymorphism) in the gene of the enzyme Stearoyl-CoA-Desaturase in Bubalus bubalis
(2007-12-01)
The milk is an important food because it contents Conjugated Linoleic Acids (CLA). These fatty acids are synthesized in mammary gland under action of the enzyme Stearoyl CoA-Desaturase (SCD) and have showed some positive ...
Diagnóstico y tipificación del virus de la leucosis bovina mediante una prueba de PCR-RFLP a partir de ADN extraído desde células somáticas de la leche
(Facultad de Ciencias Veterinarias, Universidad Austral de Chile, 2006)
Identification of new flagellin-encoding fliC genes in Escherichia coli isolated from domestic animals using RFLP-PCR and sequencing methodsIdentificação de novas flagelinas codificadas por fliC em Escherichia coli isoladas de animais domésticos utilizando RFLP-PCR e sequenciamento
(Colégio Brasileiro de Patologia Animal - CBPA, 2013)
Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene
(Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), 2008-05-01)
O objetivo deste trabalho foi genotipar o polimorfismo de nucleotídeo único (single nucleotide polymorphism - SNP) A2959G (AF159246) do gene CAST bovino, pela técnica de PCR-RFLP, e reportar a sua utilização pela primeira ...