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Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen
(2016-01-01)
Frozen and thawed ovine semen undergo morphological and functional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods seek to improve the quality and viability of the fertilizing ...
Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions
(2018-03-01)
The aim of this study was to evaluate the effect of four methods of sperm selection, on the integrity and stability of the plasma membrane, integrity of the acrosomal membrane and spermatic morphology in frozen/thawed ovine ...
Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality
(Elsevier Science, 2020-09-01)
Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs. We evaluated the extent of cholesterol and desmosterol incorporation ...
Effect of hydrogen peroxide on thawed ovine sperm motility
(Brazilian Coll Animal Reproduction, 2014-04-01)
Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen ...
Effect of hydrogen peroxide on thawed ovine sperm motility
(2014-01-01)
Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen ...
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death
(2017-12-01)
The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on ...
Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility
(Frontiers Media, 2021-06-24)
Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol ...