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Elucidating diversity in the class composition of the minicircle hypervariable region of Trypanosoma cruzi: New perspectives on typing and kDNA inheritance
(Public Library of Science, 2019)
Background Trypanosoma cruzi, the protozoan causative of Chagas disease, is classified into six main Discrete Typing Units (DTUs): TcI-TcVI. This parasite has around 105 copies of the minicircle hypervariable region (mHVR) ...
Diagnosis of Leishmania Using the Polymerase Chain Reaction: a Simplified Procedure for Field Work
(American Journal of Tropical Medicine and Hygiene, 1993)
Oligonucleotide primers directed to the minicircle kinetoplast DNA of Leishmania strains supported enzymatic amplification of this DNA by the polymerase chain reaction (PCR). A single product of 70 basepairs was obtained ...
Guide RNA Repertoires in the Main Lineages of Trypanosoma cruzi: High Diversity and Variable Redundancy Among Strains
(Frontiers Media, 2021-05-31)
Trypanosoma cruzi, as other kinetoplastids, has a complex mechanism of editing of mitochondrial mRNAs that requires guide RNAs (gRNAs) coded in DNA minicircles in the kinetoplast. There are many variations on this mechanism ...
Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kdna minicircles in trypanosoma cruzi i
(Public Library of Science, 2020-01)
Background Genetic exchange in Trypanosoma cruzi is controversial not only in relation to its frequency, but also to its mechanism. Parasexual genetic exchange has been proposed based on laboratory hybrids, but population ...
Genetic polymorphism of Trypanosoma cruzi bloodstream populations in adult chronic indeterminate Chagas disease patients from the E1224 clinical trial
(Oxford University Press, 2022-03)
Background: The role that the genetic diversity of natural Trypanosoma cruzi populations plays in response to trypanocidal treatment of chronic Chagas disease (CD) patients remains to be understood. We analysed the genetic ...
Simple methodology to directly genotype Trypanosoma cruzi discrete typing units in single and mixed infections from human blood samples
(Elsevier Science, 2016-09)
Different DNA markers to genotype Trypanosoma cruzi are now available. However, due to the low quantity of parasites present in biological samples, DNA markers with high copy number like kinetoplast minicircles are needed. ...
Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes
(Springer, 2009-01)
A low-stringency single-primer polymerase chain reaction (LSSP-PCR) typing procedure targeted to the intergenic regions of spliced-leader genes (SL) was designed to profile Trypanosoma cruzi I stocks from endemic regions ...
Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24sα ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
(Elsevier Science, 2006-08)
Flagellates indistinguishable from Trypanosoma cruzi were detected by microscopy in faecal samples of 2/110 Triatoma guasayana and 2/283 Triatoma garciabesi captured in a rural area of northwestern Argentina. Inoculation ...
Trypanosoma cruzi discrete typing units in Chagas disease patients with HIV co-infectionLinajes de Trypanosoma cruzi en pacientes con enfermedad de Chagas y coinfección por VIH
(Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", 2009-02)
Introducción. Las poblaciones naturales de T. cruzi han sido clasificadas en seis linajes filogenéticos o unidades de tipificación discreta: T. cruzi I, IIa, IIb, IIc, IId y IIe, que pueden jugar un rol en el tropismo ...
International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
(Public Library of ScienceGenética Molecular (GENMOL)San Francisco, Estados Unidos, 2021)