Now showing items 1-10 of 271
Isolation and characterization of a novel metalloprotease inhibitor from Bothrops alternatus snake serum
Resistance of snakes and some other animals to snake envenomation has been attributed to soluble factors present in their tissues. Here we report the isolation of a novel metalloprotease inhibitor from Bothrops alternatus ...
Molecular characterization and phylogenetic analysis of JussuMP-I: A RGD-P-III class hemorrhagic metalloprotease from Bothrops jararacussu snake venom
(Elsevier B.V., 2007-07-01)
Snake venom metalloproteases (SVMPs) embody zinc-dependent multidomain enzymes responsible for a relevant pathophysiology in envenomation. including local and systemic hemorrhage. The molecular features responsible for ...
Structural and functional characterization of neuwiedase, a nonhemorrhagic fibrin(ogen)olytic metalloprotease from Bothrops neuwiedi snake venom
(Academic Press Inc., 2000-09-15)
A fibrino(geno)lytic nonhemorrhagic metalloprotease (neuwiedase) was purified from Bothrops neuwiedi snake venom by a single chromatographic step procedure on a CM-Sepharose column, Neuwiedase represented 4.5% (w/w) of the ...
Biochemical and Functional Characterization of a Metalloprotease from the Thermophilic Fungus Thermoascus aurantiacus
(Amer Chemical Soc, 2009-10-14)
Protease production was carried out in solid state fermentation. The enzyme was purified through precipitation with ethanol at 72% followed by chromatographies in columns of Sephadex G75 and Sephacryl S100. It was purified ...
Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis
(Associação Brasileira de Divulgação Científica (ABRADIC), 2014)
Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
(Univ Federal Santa Maria, 2013-01-01)
Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig; without intestinal obstruction), treated group (Tg; submitted to intestinal obstruction and hydrocortisone treatment) and ...
Molecular and functional characterization of a new non-hemorrhagic metalloprotease from Bothrops jararacussu snake venom with antiplatelet activity
(Elsevier B.V., 2007-12-01)
BjussuMP-II is an acidic low molecular weight metalloprotease (Mr similar to 24,000 and pI similar to 6.5), isolated from Bothrops jararacussu snake venom. The chromatographic profile in RP-HPLC and its N-terminal sequence ...