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Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach
(American Society for Microbiology, 2012)
We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered ...
Comparison of Whole Genome Sequencing versus Standard Molecular Diagnostics for Species Identification in the Leishmania Viannia Subgenus.
(American Journal of Tropical Medicine and Hygiene, 2021)
The prognosis and treatment of New World tegumentary leishmaniasis is dependent on the infecting species, yet such species identification in the Leishmania Viannia subgenus poses a diagnostic challenge. Currently, speciation ...
From population to genome: Ecogenetics of Leishmania (Viannia) braziliensis and L (V) peruviana
(Taylor and Francis, 1995)
The size polymorphism of nine chromosomes, recognized by specific probes, was analysed in populations of Leishmania (Viannia) braziliensis and L. (V.) peruviana from various Peruvian biogeographical units. Interpretation ...
Influence of Leishmania RNA Virus 1 on Proinflammatory Biomarker Expression in a Human Macrophage Model of American Tegumentary Leishmaniasis
(Oxford University Press, 2017)
Backgound. Species of the Leishmania Viannia (L. V.) subgenus harbor the double-stranded Leishmania RNA virus 1 (LRV-1), previously identified in isolates from Brazil and Peru. Higher levels of LRV-1 in metastasizing strains ...
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of hamsterMigración de Leishmania (Viannia) panamensis y su persistencia en piel sana de hamster
(Universidad de Ciencias Aplicadas y Ambientales U.D.C.A, 2014)
Polymerase chain reaction detection of leishmania kDNA from the urine of Peruvian patients with cutaneous and mucocutaneous leishmaniasis
(American Society of Tropical Medicine and Hygiene, 2011)
We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase ...
Molecular identification of Leishmania spp. DNA from archived Giemsa-Stained slides of patients from Salta, Argentina
(American Society of Tropical Medicine and Hygiene, 2018-11)
Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. Leishmania spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through ...
Are cytochrome b gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina?
(American Society of Tropical Medicine and Hygiene, 2006-12)
Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. ...
A FRET-Based Real-Time PCR Assay to Identify the Main Causal Agents of New World Tegumentary Leishmaniasis
(Public Library of Science, 2013)
In South America, various species of Leishmania are endemic and cause New World tegumentary leishmaniasis (NWTL). The correct identification of these species is critical for adequate clinical management and surveillance ...