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Trapping of DNA radicals with the nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide and genotoxic damage: Recent advances using the immuno-spin trapping technology
(Elsevier Science Inc, 2019-10)
Immuno-spin trapping detection of DNA radicals with the nitrone spin trap 5,5-dimethyl-1-pyrrloine N-oxide (DMPO) has made important contributions towards the understanding of DNA radicalization and genotoxicity at sites ...
Spin Trapping: An Essential Tool for the Study of Diseases Caused by Oxidative Stress
(Bentham Science Publ, 2015)
Electron spin resonance (ESR), called also electron paramagnetic resonance (EPR) together with the spin trapping technique, has allowed us to study and understand how free radicals are involved in various pathologies. In ...
Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
(Elsevier Science, 2014-02)
Background: Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance ...
In Vivo Detection of Free Radicals using Molecular MRI and Immuno-Spin-Trapping in a Mouse Models for Amyotrophic Lateral Sclerosis (ALS)
(Elsevier, 2013-10)
Free radicals associated with oxidative stress play a major role in amyotrophic lateral sclerosis (ALS). By combining immuno-spin trapping and molecular magnetic resonance imaging, in vivo trapped radical adducts were ...
Combined molecular MRI and immuno-spin trapping for in vivo detection of free radicals in orthotopic mouse GL261 glioma
(Elsevier, 2013-12)
Free radicals play a major role in gliomas. By combining immuno-spin-trapping (IST) and molecular magnetic resonance imaging (mMRI), in vivo levels of free radicals were detected within mice bearing orthotopic GL261 gliomas. ...
Myeloperoxidase-induced genomic DNA-centered radicals
(American Society For Biochemistry And Molecular Biology, 2010-06)
Myeloperoxidase (MPO) released by activated neutrophils can initiate and promote carcinogenesis. MPO produces hypochlorous acid (HOCl) that oxidizes the genomic DNA in inflammatory cells as well as in surrounding epithelial ...
Radicalization of Glyceraldehyde-3-Phosphate Dehydrogenase by HOCl in Living Cells
(OMICS International, 2015-12)
A number of post-translational oxidative modifications of the enzyme “cell-redox sensor” glyceraldehyde-3- phosphate dehydrogenase (GAPDH) have been reported. These modifications affect GAPDH structure, function, and cell ...
Free radical-operated proteotoxic stress in macrophages primed with lipopolysaccharide
(Elsevier Science Inc., 2012-07)
The free-radical-operated mechanism of death of activated macrophages at sites of inflammation is unclear, but it is important to define it in order to find targets to prevent further tissue dysfunction. A well-defined ...