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Horseradish peroxidase production from Spodoptera frugiperda larvae: A simple and inexpensive method
(Elsevier, 2010-06)
Horseradish peroxidase is used in many biotechnological fields including diagnostics, biocatalysts and biosensors. Horseradish peroxidase isozyme C (HRPC) was extracellularly expressed in Spodoptera frugiperda Sf9 cell ...
Recombinant horseradish peroxidase production in species of lepidoptera frequently found in Argentina
(Elsevier Science, 2010-12)
Horseradish peroxidase isozyme C (HRPC) is an important commercial biocatalyst. In this study, a screening of different lepidopteran species frequently found in Argentina to produce this protein was carried out. Two ...
Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor
(Elsevier Science, 2005-03)
The roles of chemical kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are ...
Immobilization of horseradish peroxidase in Ca-alginate beads: Evaluation of the enzyme leakage on the overall removal of an azo-dye and mathematical modeling
(Institution of Chemical Engineers, 2021-12)
Horseradish peroxidase (HRP) was immobilized in calcium alginate beads by extruding an alginate/HRP solution through a syringe onto calcium chloride. The aim of this study was to evaluate their use to remove the azo-dye ...
Horseradish peroxidase-mediated decolourization of Orange II: modelling hydrogen peroxide utilization efficiency at different pH values
(Springer Heidelberg, 2018-05-09)
Enzymatic decolourization of azo-dyes could be a cost-competitive alternative compared to physicochemical or microbiological methods. Stoichiometric and kinetic features of peroxidase-mediated decolourization of azo-dyes ...
Extractive purification of recombinant peroxidase isozyme c from insect larvae in aqueous two-phase systems
(Elsevier Science, 2012-09)
Aqueous two-phase systems (ATPS) have not yet been applied to purify proteins expressed in insect larvae infected by recombinant baculovirus. This work describes the behavior of typical contaminants in the baculovirus-insect ...
Genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography
(Elsevier Science, 2005-09-10)
An engineered horseradish peroxidase isozyme C (HRP C) gene was constructed by the addition of a 6xArg fusion tail to 6xHis-HRP C by the PCR strategy. The 6xHis-6xArg-HRP C cDNA was expressed in the Sf9 insect cell line ...
New bioanalytical platform based on the use of avidin for the successful exfoliation of multi-walled carbon nanotubes and the robust anchoring of biomolecules. Application for hydrogen peroxide biosensing
(Elsevier Science, 2019-08)
We are reporting an innovative building-block for the development of biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with avidin (MWCNTs-avidin). In this work, at variance ...
High-level expression and purification of recombinant horseradish peroxidase isozyme C in SF-9 insect cell culture
(Elsevier, 2005-02)
A method to obtain high-expression levels of recombinant horseradish peroxidase isozyme C (HRP C) in Spodoptera frugiperda Sf-9 cell culture and a strategy for its purification are described. HRP C was secreted into the ...
Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system
(Elsevier Science, 2003-11)
The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)2 ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and ...