Now showing items 1-10 of 1498
Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
(Pontificia Universidad Católica de Valparaíso, 2008)
Molecular and Kinetic Characterization of Two Extracellular Xylanases Isolated from Leucoagaricus gongylophorus
(Humana Press Inc, 2014-06-01)
In this work, the xylanolytic profile of Leucoagaricus gongylophorus was studied, and two extracellular enzymes with xylanolytic activity (XyLg1 and XyLg2) were isolated, purified, and characterized. XyLg1 has a molecular ...
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine
Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of nucleosides and deoxynucleosides, generating ribose 1-phosphate and the purine base, which is an important step of purine catabolism pathway. ...
Lipid Metabolizing Enzyme Activities Modulated by Phospholipid Substrate Lateral Distribution
Biological membranes contain many domains enriched in phospholipid lipids and there is not yet clear explanation about how these domains can control the activity of phospholipid metabolizing enzymes. Here we used the surface ...
Occurrence of dead core in catalytic particles containing immobilized enzymes: analysis for the Michaelis–Menten kinetics and assessment of numerical methods
In this article, the occurrence of dead core in catalytic particles containing immobilized enzymes is analyzed for the Michaelis–Menten kinetics. An assessment of numerical methods is performed to solve the boundary value ...
Purification and characterization of xylanases from Aspergillus giganteus
A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. ...
HOMOGENEOUS ENZYME KINETICS
The study of enzyme kinetics is important from a fundamental scientific perspective, since it allows the formulation of molecular models for enzyme action, and also for technological reasons, since it allows the formulation ...