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Revealing a new fluorescence peak of the enhanced green fluorescent protein using three-dimensional fluorescence spectroscopy
(Royal Soc Chemistry, 2019-08-01)
Fluorescent proteins have many applications as biomarkers and biosensors in the medical and biological fields. Their success was largely supported by modifications of the first isolated fluorescent protein, the wild-type ...
Efficient transgene expression in IVF and parthenogenetic bovine embryos by intracytoplasmic injection of DNA-liposome complexes
(Wiley, 2011-04)
Transgenic animals constitute an important tool with many biotechnological applications. Although there have been advances in this field, we propose a novel method that may greatly increase the efficiency of transgenic ...
Survival in soil and detection of co-transformed Trichoderma harzianum by nested PCR.
(Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 4, p.403-405, abr. 2004., 2004)
Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)
(Springer, 2014-02)
Abstract The aim of this work was to improve existing transformation protocols and to transform specific genotypes of Paspalum notatum (bahiagrass) for functional analyses of candidate genes involved in reproduction. ...
Multiple gene knock-down by a single lentiviral vector expressing an array of short hairpin RNAs
(Pontificia Universidad Católica de Valparaíso, 2006)
Production of chimeric embryos by aggregation of bovine egfp eight-cell stage blastomeres with two-cell fused and asynchronic embryos
(Elsevier Science Inc, 2013-06)
Embryo disaggregation allows the production of two to four identical offspring from a single cow embryo. In addition, embryo complementation has become the technique of choice to demonstrate the totipotency of embryonic ...
Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector
(FUNPEC-EDITORARIBEIRAO PRETO, 2012)
Hemophilia A is the most common X-linked bleeding disorder; it is caused by deficiency of coagulation factor VIII (FVIII). Replacement therapy with rFVIII produced from human cell line is a major goal for treating hemophilia ...
Production of IVF transgene-expressing bovine embryos using a novel strategy based on cell cycle inhibitors
(Elsevier Science Inc, 2012-07)
The objective was to evaluate the effects of cell cycle inhibitors (6-dimethylaminopurine [DMAP], and dehydroleukodine [DhL]) on transgene expression efficiency and on mosaic expression patterns of IVF bovine zygotes ...