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Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis
(Elsevier Ireland, 2019-02)
We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution ...
New Perspectives in Mass Disaster Victim Identification Assisted by DNA Typing and Forensic Genomics
(Academic Press Inc Elsevier Science, 2017)
Mass disasters represent a challenging forensic task when victims need to be identified. A wide variety of multiple scenarios force a systematic classification of disasters. The retrieval of human remains, leading to the ...
Human repetitive and unique sequences coexist in a large circulating DNA species found in cryoprecipitates from SLE patients
(Clinical & Experimental Immunology, 2017)
Evaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non-virulent Paracoccidioides brasiliensis isolates
(2002-07-12)
Randomly amplified polymorphic DNA (RAPD) analysis of 35 Paracoccidioides brasiliensis isolates was carried out to evaluate the correlation of RAPD profiles with the virulence degree or the type of the clinical manifestations ...
Evaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non-virulent Paracoccidioides brasiliensis isolates
(2002-07-12)
Randomly amplified polymorphic DNA (RAPD) analysis of 35 Paracoccidioides brasiliensis isolates was carried out to evaluate the correlation of RAPD profiles with the virulence degree or the type of the clinical manifestations ...
Molecular characterization of Acidithiobacillus ferrooxidans and A-thiooxidans strains isolated from mine wastes in Brazil
(Springer, 2001-10-01)
Nineteen strains of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, including 12 strains isolated from coal, copper, gold and uranium mines in Brazil, strains isolated from similar sources in other countries ...
Novel properties of melanins include promotion of DNA strand breaks, impairment of repair, and reduced ability to damage DNA after quenching of singlet oxygen
(ELSEVIER SCIENCE INCNEW YORK, 2012)
Melanins have been associated with the development of melanoma and its resistance to photodynamic therapy (PDT). Singlet molecular oxygen (102), which is produced by ultraviolet A solar radiation and the PDT system, is ...
Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens
(2021-10-01)
Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and ...