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Improved DNA extraction technique from clot for the diagnosis of Chagas disease
(Public Library of Science, 2019)
BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater ...
Are DNA Test Infallible?
(Elsevier, 2003-01)
Modern technology has strongly influenced most fields of knowledge and forensic sciences do not escape this reality. With the advent of the application of molecular biology to human identification by means of DNA typing, ...
Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis
(Elsevier Ireland, 2019-02)
We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution ...
An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples
(Public Library of Science, 2020)
Tuberculosis (TB) is a major global public health problem with high mortality and morbidity. In low-middle income countries (LMIC) a large number of respiratory symptomatic cases that require TB screening per year demands ...
PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREESProposta de protocolo para extração de DNA de Jabuticabeira
(Universidade Federal de Santa Maria, 2011)
Evaluation of DNA fragmentation in dog sperm using the sperm chromatin dispersion test
(Wiley Blackwell Publishing, Inc, 2022-04-26)
The study's objective was to adapt the Sperm Chromatin Dispersion (SCD) protocol to evaluate sperm DNA fragmentation and implement a fragmentation control in dogs. Correlation between DNA status and routine sperm parameters ...
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp Infections
(Public Library of Science, 2016)
Cutaneous and mucosal leishmaniasis is widely distributed in Central and South America. Leishmania of the Viannia subgenus are the most frequent species infecting humans. L. (V.) braziliensis, L. (V.) panamensis are also ...
Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
(Elsevier, 2015)
An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and ...
Polymerase chain reaction detection of leishmania kDNA from the urine of Peruvian patients with cutaneous and mucocutaneous leishmaniasis
(American Society of Tropical Medicine and Hygiene, 2011)
We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase ...
The spectrum of tuberculosis described as differential DNA methylation patterns in alveolar macrophages and alveolar T cells
(BioMed Central, 2022)
Background: Host innate immune cells have been identified as key players in the early eradication of Mycobacterium tuberculosis and in the maintenance of an anti-mycobacterial immune memory, which we and others have shown ...