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Molecular detection of Clostridium difficile on inert surfaces from a Costa Rican hospital during and after an outbreak
(2016-12-01)
Background: Hospital transmission of Clostridium difficile is fostered by contamination of surfaces and
medical equipment with spores highly resistant to disinfectants and regular cleaning procedures. Despite
the outbreaks ...
Helicobacter pylori in a Costa Rican dyspeptic patient population
(1994-03)
Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded ...
Detection of shigella in lettuce by the use of a rapid molecular assay with increased sensitivity
(Brazilian Journal of Microbiology (2010) 41: 993-1000, 2010)
A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was ...
Molecular Detection of Bartonella Species in Fleas Collected from Dogs and Cats from Costa Rica
(2015-09)
The bacterial genus Bartonella includes several species with zoonotic potential, some of which are common in domestic dogs and cats, as well as in their fleas. Because there is no previous information about the presence ...
Disease-associated CAG·CTG triplet repeats expand rapidly in non-dividing mouse cells, but cell cycle arrest is insufficient to drive expansion
(Nucleic Acids Research 42(11) p. 7047–7056, 2014-05-01)
Genetically unstable expanded CAG·CTG trinucleotide repeats are causal in a number of human disorders, including Huntington disease and myotonic dystrophy type 1. It is still widely assumed that DNA polymerase slippage ...