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Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors
(1993)
DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase ...
Cloning and characterization of a DNA polymerase β gene from Trypanosoma cruzi
(2009)
A gene coding for a DNA polymerase β from the Trypanosoma cruzi Miranda clone, belonging to the TcI lineage, was cloned (Miranda Tcpolβ), using the information from eight peptides of the T. cruzi β-like DNA polymerase ...
Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form
(Springer, 2015)
Chagas disease, caused by the protozoan
Trypanosoma cruzi, is a major parasitic disease that affects
millions of people in America. However, despite the high
impact of this disease on human health, no effective and ...
Colorimetric detection of DNA polymerase activity after sodium dodecyl sulfate polyacrylamide gel electrophoresis
(1994)
A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- ...
Effect of benzo[a]pyrene on DNA synthesis and DNA polymerase activity of rat liver nuclei
(1985)
When benzo[a]pyrene (B[a]P) was administered intraperitoneally to rats 48 hr before they were killed, the DNA-synthesizing capability of isolated rat liver nuclei was decreased as compared with control animals. B[a]P also ...
Endogenous overexpression of an active phosphorylated form of DNA polymerase beta under oxidative stress in Trypanosoma cruzi
(Public Library Science, 2018)
Trypanosoma cruzi is exposed during its life to exogenous and endogenous oxidative stress, leading to damage of several macromolecules such as DNA. There are many DNA repair pathways in the nucleus and mitochondria ...
DNA evidence of Trypanosoma cruzi in the Chilean wild vector Mepraia spinolai (Hemiptera : Reduviidae)
(FUNDACO OSWALDO CRUZ, 2005-05)
Molecular evidence showed 46.2% of Trypanosoma cruzi infection in Mepraia spinolai insects from North-Central Chile, which is significantly higher than previous reports of up to 26% by microscopic observation. Our results ...
FACTORS INVOLVED IN SPECIFIC TRANSCRIPTION BY MAMMALIAN RNA POLYMERASE-II - ROLE OF FACTOR-IID AND FACTOR-MLTF IN TRANSCRIPTION FROM THE ADENOVIRUS MAJOR LATE AND IVA2 PROMOTERS
(AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC,, 2010-11-16)
The role of the adenovirus major late upstream transcription from the adenovirus major late and the IV a2 promoters was studied. The transcription initiation site of the IVa2 promoter is located 210 nucleotides upstream ...
Use of polymerase chain reaction (PCR) and hybridization assays to detect trypanosoma cruzi in chronic chagasic patients treated with itraconazole or allopurinol
(2004)
The presence of Trypanosoma cruzi in chronic chagasic patients with negative xenodiagnosis (XD) after 6 years following completion of therapy with either itraconazole or allopurinol was assessed by polymerase chain reaction ...
Genotyping of Mitochondrial Aldehyde Dehydrogenase Locus of Native American Indians
(1990)
Using the polymerase chain reaction to amplify genomic DNA from hair roots, we have examined the mitochondrial aldehyde dehydrogenase (ALDH2) genotypes of 28 individuals from the South American Mapuche Indians. We have ...