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Fluorescence resonance energy transfer and molecular modeling studies on 4 ',6-diamidino-2-phenylindole (DAPI) complexes with tubulin

Arbildua, José J.; Brunet, Juan E.; Jameson, David M.; López, Maribel; Nova Martínez, Esteban; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (COLD SPRING HARBOR LAB PRESS, 2006-03)

The goal of this work was to determine the binding properties and location of 4',6-diamidino-2-phenyl=indole (DAPI) complexed with tubulin. Using fluorescence anisotropy, a dissociation constant of 5.2 +/- 0.4 mu M for the ...

Inhibition of Tubulin Self-Assembly and Tubulin-Colchicine GTPase Activity by Guanosine 5’-γ-Fluorotriphosphate)

Monasterio Opazo, Octavio; Timasheff, Serge N. (1987)

The inhibitory effects of guanosine 5'-(ᵧ-fluorotriphosphate) [GTP(ᵧF)] on both the polymerization and the colchicine-dependent GTPase activity of calf brain tubulin have been studied. The results demonstrate that this ...