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Biotechnological applications of occlusion bodies of Baculoviruses
(Springer, 2018-08)
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main ...
Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing
(John Wiley & Sons Inc, 2019-08)
The clustered regularly interspaced short palindromic repeats (CRISPR) system?associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored ...
High-throughput cloning and expression of human ABC transporters in Baculovirus/Insect Cell system customized for X-ray crystallography studies
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2018)
Biotechnological applications of occlusion bodies of Baculoviruses
(Springer, 2018-08)
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main ...
Expressão de proteína antiviral de lonomia obliqua em sistema baculovírus/célula de inseto
(Universidade Federal de São CarlosBRUFSCarPrograma de Pós-Graduação em Biotecnologia - PPGBiotec, 2012-02-16)
In recent years, the technology animal cells culture has allowed the development of many byproducts, especially those with pharmacological interest. Some of these products, the recombinant proteins, can be produced by ...
Expression of the hemagglutinin HA1 subunit of the equine influenza virus using a baculovirus expression systemExpresión de la subunidad HA1 del virus de influenza equina utilizando un sistema de expresión en baculovirus
(Asociación Argentina de Microbiología, 2013-10)
Equine influenza virus is a leading cause of respiratory disease in horses worldwide. Disease prevention is by vaccination with inactivated whole virus vaccines. Most current influenza vaccines are generated in embryonated ...
Comparison of Strategies for the Production of FMDV Empty Capsids Using the Baculovirus Vector System
(Springer, 2014-06)
Recombinant FMDV empty capsids have been produced in insect cells and larvae using the baculovirus expression system, although protein yield and efficiency of capsid assembly have been highly variable. In this work, two ...
Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ- baculovirus occlusion and larval per os infectivity
(Elsevier Science, 2010-01)
Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign ...