Search
Now showing items 1-10 of 638
The Bias Associated with Amplicon Sequencing Does Not Affect the Quantitative Assessment of Bacterial Community Dynamics
(Public Library of Science, 2014-06)
The performance of two sets of primers targeting variable regions of the 16S rRNA gene V1-V3 and V4 was compared in their ability to describe changes of bacterial diversity and temporal turnover in full-scale activated ...
16S rRNA gene amplicon dataset of prokaryotic communities from a subantarctic marine ecosystem: Ushuaia Bay and surrounding waters
(Elsevier Inc, 2020-10)
We provide a 16S rRNA gene dataset of prokaryotic assemblages of a subantarctic marine ecosystem. Samples were collected at 2 stations (one near Ushuaia Bay and the other close to Bridges islands in the Beagle Channel). ...
Herpes simplex virus type 1-based amplicon vectors for fundamental research in neurosciences and gene therapy of neurological diseases
(Elsevier, 2012-01)
Somatic manipulation of the nervous system without the involvement of the germinal line appears as a powerful counterpart of the transgenic strategy. The use of viral vectors to produce specific, transient and localized ...
Amplicon metagenomic analysis of fungi from Antarctic Terrestrial habitats
(Frontiers Media SA, 2017)
In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools ...
An exploration tool for quality analysis in targeted sequencing experiments
(Springer, 2015-01)
Amplicon Exome Sequencing allows focusing on exonic regions of a small group of genes. The overall aim is to inquire sequenced regions about the occurrence of mutations, single nucleotide polymorphisms, insertions and ...
Deep sequencing to detect diversity of Trypanosoma cruzi infection in patients co-infected with HIV and Chagas disease
(Oxford University Press, 2021)
Chagas disease, caused by Trypanosoma cruzi, can reactivate and cause severe acute disease in immunocompromised patients such as those infected with HIV. We conducted amplicon deep sequencing of a 327-base pair fragment ...
Amplicon-metagenomic analysis of fungi from antarctic terrestrial habitats
(Frontiers Media S.A., 2017)
© 2017 Baeza, Barahona, Alcaíno and Cifuentes. In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. ...
Multiplex Amplicon Sequencing For Microbe Identification In Community-based Culture Collections
(Nature Publishing Group, 2016)
Nanopore sequencing of long ribosomal DNA amplicons enables portable and simple biodiversity assessments with high phylogenetic resolution across broad taxonomic scale
(Oxford University Press, 2019)
BACKGROUND: In light of the current biodiversity crisis, DNA barcoding is developing into an essential tool to quantify state shifts in global ecosystems. Current barcoding protocols often rely on short amplicon sequences, ...