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Alkaline phosphatases: In silico study on the catalytic effect of conserved active site residues using human placental alkaline phosphatase (plap) as a model protein
(American Chemical Society, 2020-12)
The metalloenzymes from the alkaline phosphatase (AP) superfamily catalyze the hydrolysis and transphosphorylation of phosphate monoesters. The role of several amino acids highly conserved in the active site of this family ...
Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R-prolixus (Hemiptera, Triatominae)
(Funpec-editora, 2016-01-01)
Alkaline phosphatase activity was detected in salivary gland cells of the Rhodnius neglectus Lent, 1954, and R. prolixus Stal, 1859, vectors of Trypanosoma cruzi Chagas, 1909 (etiological agent of Chagas disease) and T. ...
A new microassay for the determination of alkaline phosphatase activity in early mouse concepti
(1994)
A new enzymatic assay for the determination of alkaline phosphatase (APase) activity in preimplantation mouse concepti is described. This method allows estimation of APase activity of concepti extracts using the fluorogenic ...
Production of extracellular alkaline proteases by Aspergillus clavatus
(Springer, 2005-03-01)
The production of extracellular alkaline proteases from Aspergillus clavatus was evaluated in a culture filtrate medium, with different carbon and nitrogen sources. The fungus was cultivated at three different temperatures ...
Purification and characterization of an iron-activated alkaline phosphatase produced by rhizopus microsporus var. Microsporus under submerged fermentation using rye flour
(2020-07-01)
Current researches have been carried out to find microorganisms that can produce enzymes for different biotechnological purposes. Among the enzymes, the microbial phosphatases, responsible for hydrolyzing phosphoric acid ...
Nutrient deficiency and hypoxia as constraints to Panicum coloratum growth in alkaline soils
(Wiley; British Grassland Society; European Grassland Federation, 2017-12)
Alkaline and saline–alkaline soils impose severe restrictions on plant growth. Panicum coloratum var. coloratum is a perennial C4 forage grass widely used in tropical and subtropical environments. Published information on ...
Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...