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Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
(Elsevier, 2021-03-10)
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium ...
A metabolic pathway leading to mannosylfructose biosynthesis in Agrobacterium tumefaciens uncovers a family of mannosyltransferases
(National Academy of Sciences, 2007-09-04)
A metabolic pathway for biosynthesis of the nonreducing disaccharide mannosylfructose (β-fructofuranosyl-α-mannopyranoside), an important osmolyte in Agrobacterium tumefaciens, was discovered. We have identified and ...
Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo
(Ediciones INTA, 2020-08)
La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ...
Editorial : Plant transformation
(Frontiers Media, 2022-04)
Plant transformation provides a key tool for much basic research, such as the study of gene functions and interactions, protein–protein interactions, developmental processes, as well as applications for crop improvement ...
Endophytic microorganisms Agrobacterium tumefaciens 6N2 and Meyerozyma guilliermondii 6N serve as models for the study of microbial interactions in colony biofilms
(Asociación Argentina de Microbiología, 2019-01)
We analyzed the morphology of colonies of endophytic bacteria and yeasts by fluorescence. Agrobacterium tumefaciens 6N2 (formerly 197MX) is a non-pathogenic isolate from sugarcane with features of relevance for microbial ...
Characterization of chimeric ADPglucose pyrophosphorylases of Escherichia coli and Agrobacterium tumefaciens. Importance of the C-terminus on the selectivity for allosteric regulators
(American Chemical Society, 2002-07)
ADPglucose pyrophosphorylase catalyzes the regulatory step in the pathway for bacterial glycogen synthesis. The enzymes from different organisms exhibit distinctive regulatory properties related to the main carbon metabolic ...
Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo
(Instituto Nacional de Tecnología Agropecuaria, 2020-08)
La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal, que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ...
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
(Springer Netherlands, 2021-01-25)
Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ...
Transcription factor RF2a alters expression of the rice tungro bacilliform virus promoter in transgenic tobacco plants
(National Academy of Sciences, 2001-06)
The promoter from rice tungro bacilliform badnavirus (RTBV) is expressed only in phloem tissues in transgenic rice plants. RF2a, a b-Zip protein from rice, is known to bind to the Box II cis element near the TATA box of ...
On the simultaneous activation of Agrobacterium tumefaciens ADP-glucose pyrophosphorylase by pyruvate and fructose 6-phosphate
(Elsevier France-Editions Scientifiques Medicales Elsevier, 2020-04)
Bacterial ADP-glucose pyrophosphorylases are allosterically regulated by metabolites that are key intermediates of central pathways in the respective microorganism. Pyruvate (Pyr) and fructose 6-phosphate (Fru6P) activate ...