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Whole cell patch clamp recording of mouse and rat inner hair cells in the intact organ of Corti
(Springer, 2016)
Whole cell patch clamping is a widely applied method to record currents across the entire membrane of a cell. This protocol describes application of this method to record currents from the sensory inner hair cells in the ...
Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release
(National Academy of Sciences, 2020-02)
Synaptotagmin 1 (Syt1) synchronizes neurotransmitter release to action potentials (APs) acting as the fast Ca2+ release sensor and as the inhibitor (clamp) of spontaneous and delayed asynchronous release. While the Syt1 ...
Mouse sperm patch-clamp recordings reveal single Cl- channels sensitive to niflumic acid, a blocker of the sperm acrosome reaction
(1998)
Ion channels lie at the heart of gamete signaling. Understanding their regulation will improve our knowledge of sperm physiology, and may lead to novel contraceptive strategies. Sperm are tiny (~ 3 μm diameter) and, until ...
Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices
(Humana Press Inc., 2024)
Effects of acetylcholine and cholinergic antagonists on the activity of nucleus of the solitary tract neurons
(Elsevier B.V., 2017-03-15)
Previously we have demonstrated that microinjection of acetylcholine (ACh) into the intermediate nucleus of the solitary tract (iNTS) induced sympatho-inhibition combined with a decrease in the phrenic nerve activity (PNA), ...
Nicotinic acetylcholine receptors at the single-channel level
(Wiley Blackwell Publishing, Inc, 2018-06)
Over the past four decades, the patch clamp technique and nicotinic ACh (nACh) receptors have established an enduring partnership. Like all good partnerships, each partner has proven significant in its own right, while ...
Effect of glutamate receptor phosphorylation by endogenous protein kinases on electrical activity of isolated postsynaptic densities of rat cortex and hippocampus
(Elsevier Ireland Ltd, 1997)
Postsynaptic densities (PSDs) were isolated from rat brain cortex and hippocampus, purified and incorporated into giant (5-80 μm in diameter) liposomes. Gigaohm seals were obtained with a patch-clamp pipette, and a giant ...