Buscar
Mostrando ítems 41-50 de 6421
Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
(Inst Tecnologia Parana, 2017-01-01)
Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to ...
A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
(2016-01-01)
Groundnut ringspot virus (GRSV) is a tospovirus species transmitted in a circulative propagative manner by several thrips species. Traditionally, GRSV is detected by enzyme-linked immunosorbent assay (ELISA) and reverse ...
Oral polio vaccine in infants does not interfere in detection of enterovirus in blood
(SOC CHILENA INFECTOLOGIA, 2013)
Introduction: There is not known if a viraemia post-oral polio vaccine (OPV) is detectable by modern molecular techniques. Such viraemia could affect the performance of the real time-polymerase chain reaction (PCR) for non ...
A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
(Pontificia Universidad Católica de Valparaíso, 2011)
Report of a real-time pcr assay for paenibacillus larvae dna detection from spores of scale samplesReporte de un ensayo de PCR en tiempo real para la detección de ADN de Paenibacillus larvae a partir de esporas de muestras de escamas
(Asociación Argentina de Producción Animal, 2017-12)
The Gram-positive bacterium Paenibacillus larvae is one of the major pathogens of Apis mellifera, causing the disease knownas American foulbrood. Identification of P. larvae by microbiological methods can be difficult and ...
Quantification by real-time PCR of Trypanosoma cruzi DNA in samples of Triatoma infestans used in xenodiagnosis of chronic Chagas disease patients
(BioMed Central Ltd., 2016)
© 2016 The Author(s).Background: Trypanosoma cruzi multiplies and differentiates in the digestive tract of triatomine insects. Xenodiagnosis (XD) is a parasitological tool in which the insect vectors acts as a biological ...
Real-Time PCR in faecal samples of Triatoma infestans obtained by xenodiagnosis: Proposal for an exogenous internal control
(2012)
Background: The polymerase chain reaction (PCR) has proved to be a sensitive technique to detect Trypanosoma cruzi in the chronic phase of Chagas disease, which is characterized by low and fluctuating parasitemia. Another ...
Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples
(Springer, 2012-05-01)
Cryptosporidium parvum infection is very important with respect to public health, owing to foodborne and waterborne outbreaks and gastrointestinal illness in immunocompetent and immunocompromised persons. In cattle, infection ...