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Detection of Salmonella spp. in water using magnetic capture hybridization combined with PCR or real-time PCR
(IWA Publishing, 2006-03)
The removal of target DNA by magnetic capture hybridization (MCH) from constituents inhibitory to amplification by polymerase chain reaction (PCR) was evaluated using Salmonella as the test pathogen. Hybrids were subjected ...
Comparação entre dois métodos de detecção de DNA de papilomavírus humano em carcinoma epidermoide de lábio
(Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2010-04-01)
Introduction: Human papillomavirus (HPV) has been currently associated with oral carcinogesis. The methodology applied in virus detection is one of the main reasons for the great variability observed in HPV detection. ...
First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina
(Sociedade Brasileira de Microbiologia, 2013-11)
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle ...
Aplicação de métodos moleculares no diagnóstico de endoftalmite bacteriana
(Universidade Federal de São Paulo (UNIFESP), 2009-04-29)
Objective: Development and application of Nested Multiplex PCR and Real Time PCR assays for detection and Gram classification of bacteria using aqueous and vitreous humor collected from patients with suspected endophthalmitis. ...
Migratory route of Strongyloides venezuelensis in Lewis rats: Comparison of histological analyses and PCR
(Academic Press Inc. Elsevier B.V., 2011-02-01)
Strongyloides venezuelensis is a parasitic nematode that has been used as a model to study human and animal strongyloidiasis. In this study, we compared the sensitivity between traditional methodologies and PCR assay to ...
Migratory route of Strongyloides venezuelensis in Lewis rats: Comparison of histological analyses and PCR
(Academic Press Inc. Elsevier B.V., 2011-02-01)
Strongyloides venezuelensis is a parasitic nematode that has been used as a model to study human and animal strongyloidiasis. In this study, we compared the sensitivity between traditional methodologies and PCR assay to ...
Use of Escherichia coli BOX-PCR fingerprints to identify sources of fecal contamination of water bodies in the State of Sao Paulo, Brazil
(Academic Press Ltd- Elsevier Science LtdLondonInglaterra, 2012)
Significant performance variation among PCR systems in diagnosing congenital toxoplasmosis in São Paulo, Brazil: analysis of 467 amniotic fluid samples
(Faculdade de Medicina / USP, 2009)
INTRODUCTION: Performance variation among PCR systems in detecting Toxoplasma gondii has been extensively reported and associated with target genes, primer composition, amplification parameters, treatment during pregnancy, ...