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Clonagem e caracterização funcional do gene Rad51 de Trypanosoma cruzi
(Universidade Federal de Minas GeraisUFMG, 2002-03-01)
Trypanosoma cruzi is a parasite of the order Kinetoplastida that causesChagas disease. A low level of allele divergence is observed in this organism. Although such high levels of homozygosity are not expected in an organism ...
RADSex: A computational workflow to study sex determination using restriction site-associated DNA sequencing data
(Wiley-Blackwell, 2021-03-09)
The study of sex determination and sex chromosome organization in nonmodel species has long been technically challenging, but new sequencing methodologies now enable precise and high-throughput identification of sex-specific ...
The potential of genome-wide RAD sequences for resolving rapid radiations: a case study in Cactaceae
(2020-10-01)
The reconstruction of relationships within recently radiated groups is challenging even when massive amounts of sequencing data are available. The use of restriction site-associated DNA sequencing (RAD-Seq) to this end is ...
SNP identification and validation on genomic DNA for studying genetic diversity in Thunnus albacares and Scomberomorus brasiliensis by combining RADseq and long read high throughput sequencing
(2018-02-01)
A combination of a RADseq method (ddRAD) with long read high throughput sequencing (Roche 454) was tuned up in order to identify and validate a set of SNPs useful for gene diversity analysis in two important South American ...
Hierarchical biogeographical processes largely explain the genomic divergence pattern in a species complex of sea anemones (Metridioidea: Sagartiidae: Anthothoe)
(Academic Press Inc Elsevier Science, 2018-10)
The phylogenetic resolution provided by genome-wide data has demonstrated the usefulness of RAD sequencing to tackle long-standing taxonomic questions. Cnidarians have recently become a model group in this regard, yet ...
A RAD-based linkage map of kiwifruit (Actinidia chinensis Pl.) as a tool to improve the genome assembly and to scan the genomic region of the gender determinant for the marker-assisted breeding
(Springer, 2015)
Kiwifruit breeding still largely relies on phenotypic observation of cross progeny grown in the field to fruiting maturity, without any selection prior to the juvenility being overcome. Developing markers for the selection ...
Estudos sobre os mecanismos de recombinação em trypanosoma cruzi: obtenção e análise de células superexpressando o gene TcRaD51
(Universidade Federal de Minas GeraisUFMG, 2006-08-10)
Trypanosoma cruzi is the parasite that causes Chagas disease. Only a few genes involved in DNA metabolism have been described in this species. Recently, our group characterized a gene encoding one of the key proteins ...