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GP5+/6+ SYBR Green methodology for simultaneous screening and quantification of human papillomavirus
(ELSEVIER SCIENCE BV, 2009)
Background: Detection and quantification of human papillomavirus (HPV) may help in predicting the evolution of HPV infection and progression of associated lesions. Objectives: We propose a novel protocol using consensus ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Development of a multiplex qPCR in real time for quantification and differential diagnosis of Salmonella Gallinarum and Salmonella Pullorum
(2017-11-02)
Currently there are 2659 Salmonella serovars. The host-specific biovars Salmonella Pullorum and Salmonella Gallinarum cause systemic infections in food-producing and wild birds. Fast diagnosis is crucial to control the ...
Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I
(Japan Soc Vet Sci, 2018-04-01)
A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. ...
Isolamento, caracterização molecular e detecção por PCR em tempo real (qPCR) de Salmonella spp. em amostras fecais de potros com e sem diarreia.
(Universidade Estadual Paulista (Unesp), 2021-11-29)
O presente estudo investigou a ocorrência de Salmonella spp. em fezes de 200 potros com até 1 ano de idade, através das técnicas de cultivo bacteriológico e PCR em tempo real (qPCR) pelos métodos de corante fluorescente ...