Otro
Comparison of diferent diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis
Registro en:
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 17, n. 3, p. 348-352, 2011.
1678-9180
1678-9199
10.1590/S1678-91992011000300015
S1678-91992011000300015
WOS:000294438900014
S1678-91992011000300015-en.pdf
2-s2.0-80052735361
Autor
Sassaki, C. Y.
Colodel, M. M.
Ferreira, I.
Nogueira, F. S.
Lucheis, Simone Baldini
Langoni, Hélio
Rocha, Noeme Sousa
Resumen
Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofuorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more efective than PCR of lymph node aspirate culture. There was no statistical diference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node aspirate culture and PCR of hemoculture. All infected and uninfected animals had positive and negative results in at least one test. In conclusion, the association of several tests improves the efcacy of canine visceral leishmaniasis diagnosis. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)