Tissue inhibitor of metalloproteinase-2 (TIMP-2) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDelella, Flávia K.
dc.creatorJustulin Jr., Luis A.
dc.creatorFelisbino, Sérgio L.
dc.date2014-05-27T11:22:24Z
dc.date2016-10-25T18:23:36Z
dc.date2014-05-27T11:22:24Z
dc.date2016-10-25T18:23:36Z
dc.date2007-03-01
dc.date.accessioned2017-04-06T01:23:51Z
dc.date.available2017-04-06T01:23:51Z
dc.identifierCell Biology International, v. 31, n. 3, p. 229-234, 2007.
dc.identifier1065-6995
dc.identifierhttp://hdl.handle.net/11449/69536
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69536
dc.identifier10.1016/j.cellbi.2006.10.004
dc.identifier2-s2.0-33847022254
dc.identifierhttp://dx.doi.org/10.1016/j.cellbi.2006.10.004
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890766
dc.descriptionMatrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a major role in extracellular matrix component degradation in several normal and abnormal tissue situations; they are also found in human seminal plasma. MMPs have been found in rat prostate secretions and are nearly lobe specific in expression pattern. The aim of this study was to evaluate whether TIMP-2, like other semen components, is expressed differently from different rat prostatic lobes. Immunohistochemical staining was performed in both young and adult rat ventral (VP), lateral (LP), dorsal (DP), and anterior (AP) prostatic lobes and confirmed by western blotting. TIMP-2 expression was found in the epithelial cells in the following sequence: LP > AP > DP > VP, in both young and adult rats. In this study, 100% of adult LP presented histological signs of prostatitis, where TIMP-2 immunostaining was positive in normal epithelium even with intraluminal neutrophils, but was reduced or absent in the epithelium with intraepithelial leukocytes or with periductal stroma disorganization associated with mononuclear cell infiltration. However, TIMP-2 expression in LP was not induced by prostatitis, since younger rat LPs were also strongly TIMP-2 positive. The distal and intermediate VP regions were TIMP-2 negative, but the proximal regions were strongly stained. Western blotting results confirmed the high TIMP-2 expression in the LP lobe. Thus, TIMP-2 is expressed differently between the prostatic lobes and is another nearly lobe-specific protein, which plays a role in the regulation of MMP activity in seminal plasma and glandular homeostasis. TIMP-2 is also another regional ductal variation of VP. Further studies should address whether TIMP-2 expression is related to the highest incidence of rat LP prostatitis and adenocarcinoma. © 2006 International Federation for Cell Biology.
dc.languageeng
dc.relationCell Biology International
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCollagen fiber
dc.subjectMetalloproteinase
dc.subjectProstate
dc.subjectProstatitis
dc.subjectSeminal plasma
dc.subjectTissue inhibitor of metalloproteinase
dc.subjectmatrix metalloproteinase
dc.subjecttissue inhibitor of metalloproteinase 2
dc.subjectadenocarcinoma
dc.subjectanimal tissue
dc.subjectcell separation
dc.subjectcontrolled study
dc.subjectenzyme regulation
dc.subjecthomeostasis
dc.subjectimmunohistochemistry
dc.subjectleukocyte
dc.subjectmale
dc.subjectmononuclear cell
dc.subjectneutrophil
dc.subjectnonhuman
dc.subjectprostate
dc.subjectprostate anterior lobe
dc.subjectprostate dorsal lobe
dc.subjectprostate epithelium
dc.subjectprostate lateral lobe
dc.subjectprostate ventral lobe
dc.subjectprostatitis
dc.subjectprotein analysis
dc.subjectprotein content
dc.subjectprotein expression
dc.subjectprotein induction
dc.subjectprotein localization
dc.subjectrat
dc.subjectregulatory mechanism
dc.subjectseminal plasma
dc.subjectstroma cell
dc.subjectWestern blotting
dc.subjectAging
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectImmunohistochemistry
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectTissue Inhibitor of Metalloproteinase-2
dc.subjectRattus
dc.titleTissue inhibitor of metalloproteinase-2 (TIMP-2) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry
dc.typeOtro


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