Artículo de revista
Isolation and Molecular characterization of microorganisms with potential for the degradation of oil and grease from palm oil refinery wastes
Fecha
2016-04Registro en:
Rodriguez Z., Agualimpia B., Zafra G., 2016, Isolation and molecular characterization of microorganisms with
potential for the degradation of oil and grease from palm oil refinery wastes, Chemical Engineering Transactions, 49, 517-522
DOI: 10.3303/CET1649087
10.3303/CET1649061
978-88-95608-40-2
2283-9216
Autor
Rodríguez Mateus, Zully
Agualimpia Valderrama, Bayron-Enrique
Zafra, German
Institución
Resumen
In this study we isolated and characterized native microorganisms with the ability to degrade oil and grease
(O&G) and evaluated their potential for the treatment of POMEs from a palm oil refining process. Yeast and
bacterial isolates were obtained from solid and liquid wastes from a grease trap of a palm oil refining process,
based on their ability to use palm oil as sole carbon source in solid medium. Molecular identification of
microorganisms was performed by PCR techniques, revealing that isolates corresponded to Candida and
Bacillus species, with a high degree of similarity with reported O&G-degrading organisms. Five out of these
isolates showed lipolytic activity evidenced by changes in the turbidity, colour and produced a substantial
decrease in O&G concentrations in liquid MBS cultures containing palm oil. These isolates promoted the
highest O&G decrease in POME samples with 56 %, 77 %, 78 %, 76 % and 79 % O&G removal after 72 hours
respectively. A microbial consortia composed of five degrading yeasts produced a O&G reduction up to 84 %
in POME samples after 48 hours, evidencing a synergic effect of the microorganisms. The results of this study
showed that bioaugmentation of polluted wastewaters from palm oil extraction with native microorganisms
isolated from oily residues can be efficiently used to greatly improve the removal of grease, oils and organic
matter.